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Series GSE215244 Query DataSets for GSE215244
Status Public on Jun 09, 2023
Title Divergent Functions of Histone Acetyltransferase Paralogs KAT2A and KAT2B in Keratinocyte Stemness and Differentiation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The mammalian epidermis undergoes constant renewal replenished by a pool of stem cells and terminal differentiation of their progeny. This is accompanied by changes in gene expression and morphology orchestrated, in part, by epigenetic modifiers. Here, we defined the role of histone acetyltransferase KAT2A in epidermal homeostasis and provided a comparative analysis that revealed key functional divergence with its paralogue, KAT2B. In contrast to KAT2B's reported function in epidermal differentiation, KAT2A supports the undifferentiated state in keratinocytes. RNA-seq analysis of KAT2A- and KAT2B- depleted keratinocytes revealed dysregulated epidermal differentiation. Depletion of KAT2A led to premature expression of epidermal differentiation genes in the absence of inductive signals, whilst loss of KAT2B delayed differentiation. KAT2A acetyltransferase activity was indispensable in regulating epidermal differentiation gene expression. The metazoan-specific N-terminus of KAT2A was also required to support its function in keratinocytes. We further showed that the interplay between KAT2A- and KAT2B- mediated regulation was important for normal cutaneous wound healing in vivo. Overall, these findings reveal a distinct mechanism in which keratinocytes utilize a pair of highly homologous histone acetyltransferases to support divergent functions in self-renewal and differentiation processes.
Overall design RNA-seq
Web link
Contributor(s) Walters BW, Lim CY, Tan C, Dube CT, Lee K, Koh J, Ong Y, Fathima Shah Jahan R, Tan V, Tan T
Citation(s) 37259855
Submission date Oct 11, 2022
Last update date Jun 09, 2023
Contact name Chin Yan Lim
Organization name A*STAR Skin Research Labs
Street address 8A Biomedical Grove
City Singapore
State/province Singapore
ZIP/Postal code 138648
Country Singapore
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (24)
GSM6630312 RME816
GSM6630313 RME817
GSM6630314 RME818
BioProject PRJNA889419

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE215244_DESeq2_Normalized_Counts.csv.gz 2.0 Mb (ftp)(http) CSV
GSE215244_metadata.csv.gz 266 b (ftp)(http) CSV
GSE215244_raw_counts.csv.gz 780.9 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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