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Series GSE21528 Query DataSets for GSE21528
Status Public on Mar 22, 2011
Title Induction of a peptide with activity against a broad spectrum of pathogens in the Aedes aegypti salivary gland, following infection with dengue virus
Organism Aedes aegypti
Experiment type Expression profiling by SAGE
Summary Dengue viruses (DENV) are generally maintained in a cycle which requires horizontal transmission via their arthropod vector, Ae. Aegypti, to the vertebrate host. One important consequence of this process is the interference of these arboviruses with both invertebrate and vertebrate immune systems. While infection of vertebrates causes disease, the presence of DENV gives rise to life-long, persistent infection in mosquitoes. The results of a comparative transcriptome analysis between DENV-infected and uninfected salivary glands revealed activation of both the immune deficiency (IMD) and the Toll pathways, as well as involvement of the putative antibacterial cecropin-like peptide (AAEL000598), in controlling DENV infection in Ae. aegypti. The mature form of this peptide was found to be active against DENV and Chikungunya viruses, whereas its precursor also had a strong anti-Leishmania effect. This study is the first to establish a comparative transcriptome analysis of DENV-infected and uninfected salivary glands and demonstrates that certain DENV-induced peptides, that are part of the IMD pathway, possess broad-spectrum anti-pathogenic activity and may have therapeutic potential in human.
 
Overall design Infectious blood meals were offered to 3-day-old, adult, female Ae. aegypti Liverpool mosquitoes using a silicone membrane feeder system (Alto et al., 2005). Human blood was combined with DENV-2 16681 to provide a blood meal titer of 5.106 plaque forming units (PFU)/ml. At different time-points after the blood meal, salivary glands were dissected in acid guanidium thiocyanate-phenol-chloroform (RNAble; Eurobio, France) or phosphate-buffered saline (PBS), and the samples were frozen at -70°C until use.
 
Contributor(s) Misseé D, Piquemal D
Citation(s) 21249175
Submission date Apr 27, 2010
Last update date Mar 22, 2012
Contact name David Piquemal
E-mail(s) piquemal@univ-montp2.fr
Phone +33 610430140
Organization name ACOBIOM
Street address 1682, rue de la Valsière
City Montpellier
ZIP/Postal code 34184
Country France
 
Platforms (1)
GPL10360 SAGE:17:NlaIII:Aedes aegypti
Samples (2)
GSM537746 Aedes aegypti salivary gland tags, following infection with dengue virus
GSM537747 Aedes aegypti salivary gland tags
Relations
BioProject PRJNA125945

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