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| Status |
Public on Mar 15, 2023 |
| Title |
Role of long non-coding RNA in regulatory network response to ‘Candidatus Liberibacter asiaticus’ in citrus |
| Organisms |
Citrus sinensis; Citrus jambhiri |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The goals of this study are to reveal the transcriptional and regulatory dynamics of the lncRNAs in response to CLas infection and provide a reference for a better understanding of the role of lncRNAs involved in citrus HLB regulation. For the purpose, we obtained RNA-seq-based differential expression profile of the leave midrib of CLas- and mock-inoculated rough lemon and sweet orange at week 0, 7, 17, and 34 following infections with three biological replicates in the greenhouse. Approximately 2,523 million paired-end reads from 48 sample libraries were produced using Illumina HiSeq 2000. we systematically identified lncRNAs from rough lemon and sweet orange at four different time points of a greenhouse experiment and characterized their genomic transcriptional and regulatory dynamics. We predicted their potential regulatory genes and functions and constructed a co-expression network. Our study provides precious information and expands the knowledge of citrus lncRNA against HLB disease.
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| Overall design |
mRNA profiles of the leave midrib of CLas- and mock-inoculated rough lemon and sweet orange at week 0, 7, 17, and 34.
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| Contributor(s) |
Zhuo X, Yu Q, Russo R, Zhang Y, Wei X, Wang YZ, Holden P, Gmitter FG |
| Citation(s) |
36890903 |
| Submission date |
Oct 12, 2022 |
| Last update date |
Mar 17, 2023 |
| Contact name |
Xiaokang Zhuo |
| E-mail(s) |
zhuoxiaokang@ufl.edu
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| Organization name |
University of Florida
|
| Department |
Citrus Research and Education Center
|
| Street address |
700 Experiment Station Rd
|
| City |
Lake Alfred |
| State/province |
Florida |
| ZIP/Postal code |
33850 |
| Country |
USA |
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| Platforms (2) |
| GPL17145 |
Illumina HiSeq 2000 (Citrus sinensis) |
| GPL29550 |
Illumina HiSeq 2000 (Citrus jambhiri) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA890283 |
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