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| Status |
Public on Oct 31, 2022 |
| Title |
miRNA Expression in PDGFRA Mutant GISTs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Context: Gastrointestinal stromal tumors (GISTs) harboring mutations in the PDGFRA gene occur in only about 5–7% of patients. The most common PDGFRA mutation is exon 18 D842V, which is corre-lated with specific clinico-pathological features compared to the other PDGFRA mutated GISTs. Herein, we present a miRNA expression profile comparison of PDGFRA D842V mutant GISTs and PDGFRA with mutations other than D842V (non-D842V). miRNA expression profiling was carried out on 10 patients using a TLDA miRNA array. Then, miRNA expression was followed by bioinformatic analysis aimed at evaluating differential expression, pathway enrichment, and miRNA-mRNA networks. We highlighted 24 differentially expressed miRNAs between D842V and non-D842V GIST patients. Pathway enrichment analysis showed that deregulated miRNAs targeted genes that are mainly involved in the immune response pathways. The miRNA-mRNA networks highlighted a signature of miRNAs/mRNA that could explain the indolent behavior of the D842V mutated GIST. The results highlighted a different miRNA fingerprint in PDGFRA D842V GISTs compared to non-D842Vmutated patients, which could explain the different bio-logical behavior of this GIST subset.
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| Overall design |
qPCR miRNA expression profiling. Total RNA from serum of 10 GIST patients was extracted and PDGFRA Exon 18 D842V GISTs versus PDGFRA Exon 18 non D842V GISTs
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| Contributor(s) |
Ravegnini G, Angelini S, Gorini F, Hrelia P |
| Citation missing |
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| Submission date |
Oct 13, 2022 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Gloria Ravegnini |
| Organization name |
UNIVERSITY OF BOLOGNA
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| Department |
PHARMACY AND BIOTECHNOLOGY
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| Street address |
via irnerio 48
|
| City |
Bologna |
| State/province |
BO |
| ZIP/Postal code |
40126 |
| Country |
Italy |
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| Platforms (1) |
| GPL32743 |
TaqMan Low Density Arrays (TLDA) pools A and B (Applied Biosystems) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA890223 |
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