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| Status |
Public on Jan 12, 2011 |
| Title |
Mouse bulge (CD34+CD200+CD49+) versus secondary hair germ (CD34-CD200+CD49+) versus interfollicular epidermis (CD34-CD200-CD49+) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mouse back skin was disassociated to single cells, sorted by cell surface markers and tested by microarrray
To compare the gene expression of mouse bulge (CD34+CD200+CD49+) versus secondary hair germ (CD34-CD200+CD49+) versus interfollicular epidermis (CD34-CD200-CD49+)
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Bald scalp retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells
Androgenetic alopecia (AGA) or common baldness results from a marked decrease in hair follicle size. This miniaturization may be related to loss of hair follicle stem or progenitor cells. To test this hypothesis, we analyzed bald and non-bald scalp from the same individuals for the presence of hair follicle stem and progenitor cells using flow cytometry to quantitate cells expressing CYTOKERATIN 15 (KRT15), CD200, CD34 and ALPHA-6-INTEGRIN (ITGA6). High levels of KRT15 expression correlated with stem cell properties of small cell size and quiescence. Cells with the highest level of KRT15 expression were maintained in bald scalp; however, distinct populations of CD200high ITGA6high cells and CD34-positive cells were markedly diminished. Consistent with a progenitor cell phenotype, the diminished populations localized closely to the stem-cell rich bulge area but were larger and more proliferative than the bulge stem cells. In functional assays, analogous CD200 high /Itga6 high cells from murine hair follicles were multipotent and generated new hair follicles in skin reconstitution assays. These findings suggest that a defect in stem cell activation plays a role in the pathogenesis of AGA.
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| Overall design |
4 independent biologic replicates (each pooled from 3 distinct mice) were sorted for Mouse bulge (CD34+CD200+CD49+) versus secondary hair germ (CD34-CD200+CD49+) versus interfollicular epidermis (CD34-CD200-CD49+)
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| Contributor(s) |
Garza LA, Zhou T, Blat H, Lee M, He H, Stanton DC, Carrasco L, Tobias J, Yang CC, Cotsarelis G |
| Citation(s) |
21206086 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K08 AR055666 |
The role of prostaglandin d2 in hair follicle biology |
JOHNS HOPKINS UNIVERSITY |
Luis Andres Garza |
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| |
| Submission date |
Apr 28, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Luis Andres Garza |
| E-mail(s) |
LAG@jhmi.edu
|
| Phone |
410-955-8662
|
| Organization name |
Johns Hopkins School of Medicine
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| Department |
Dermatology
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| Lab |
Garza lab
|
| Street address |
1550 Orleans St. Suite 204 CRBII Koch
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE21569 |
Bald scalp retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells |
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| Relations |
| BioProject |
PRJNA129311 |
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