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Series GSE215852 Query DataSets for GSE215852
Status Public on Dec 01, 2023
Title PTPN2 regulates metabolic flux to affect beta cell susceptibility to inflammatory stress
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Protein tyrosine phosphatase N2 (Ptpn2) is a type 1 diabetes (T1D) candidate gene identified from human genome-wide association studies. PTPN2 is highly expressed in human and murine islets and becomes elevated upon inflammation, suggesting that PTPN2 may be important for beta cell survival in the context of T1D. To test whether PTPN2 contributed to beta cell dysfunction in an inflammatory environment, we generated a beta cell-specific deletion of Ptpn2 in mice (Ptpn2 βKO). While unstressed animals exhibit normal metabolic profiles, streptozotocin (STZ) Ptpn2 βKO mice display marked increase in hyperglycemia and death due to exacerbated beta cell loss. Furthermore, cytokine treated Ptpn2 KO islets resulted in mitochondrial defects and reduced glucose-induced metabolic flux, suggesting beta cells lacking Ptpn2 are more susceptible to inflammatory stress associated with T1D due to compromised metabolic fitness.
Overall design RNA-seq of Ctrl and PTPN2-bKO islets with and without treatment of cytokines (4 total conditions).
Contributor(s) Kim YK, Kim YJ, Wells KL, Guney M, Zee T, Karsenty G, Nakayasu E, Sussel L
Citation(s) 38015772
Submission date Oct 16, 2022
Last update date Mar 01, 2024
Contact name Kristen Wells
Organization name University of Colorado Anschutz
Department Barbara Davis Center
Lab Barbara Davis Center
Street address 1775 Aurora Ct,
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM6643247 PTPN2 -/- Rep 1 not treated
GSM6643248 PTPN2 -/- Rep 2 not treated
GSM6643249 PTPN2 -/- Rep 3 not treated
BioProject PRJNA891028

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Supplementary file Size Download File type/resource
GSE215852_counts.txt.gz 1020.5 Kb (ftp)(http) TXT
GSE215852_filtered_counts.tsv.gz 745.2 Kb (ftp)(http) TSV
GSE215852_metadata.tsv.gz 331 b (ftp)(http) TSV
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