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Status |
Public on Feb 26, 2024 |
Title |
Single-cell chromatin landscape of non-myocytes reveals AP-1 as a key transcriptional regulator contributing to highly cooperative networks among non-myocyte subtypes during zebrafish heart regeneration [Cut&Tag] |
Organism |
Danio rerio |
Experiment type |
Other
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Summary |
We applied single-cell Assay for Transposase Accessible Chromatin using sequencing (scATAC-seq) to determine the intrinsic genetic programs underlying nonCM subpopulation determination during zebrafish heart regeneration at a single cell level. A total of approximately 13,000 single non-CMs derived from zebrafish hearts isolated at different time points during heart regeneration were measured and defined into diverse cell populations, exploited for cell type-specific cis-regulatory elements and enriched motifs as control catalog of downstream cell-state-determining transcription factors.
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Overall design |
To assess the dynamics of chromatin lanscape of non-CMs during zebrafish heart regenration, we collected the hearts from 0, 2, 7, 14 days post amputation and lysis the nuclei to perform scATAC-seq.
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Contributor(s) |
Dong Y, Wang H, Liu J |
Citation(s) |
38381829 |
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Submission date |
Oct 18, 2022 |
Last update date |
Feb 27, 2024 |
Contact name |
LI QIAN |
E-mail(s) |
li_qian@med.unc.edu
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Phone |
919 9624982
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Organization name |
UNC
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Department |
Department of pathology
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Lab |
Qian Lab
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Street address |
111 Mason Farm Road, MBRB
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City |
Chapel Hill |
ZIP/Postal code |
27517 |
Country |
USA |
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Platforms (1) |
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Samples (4)
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GSM6652440 |
dpi7_CUT&Tag_H3R27ac_FB:dnAP-1_replicate1 [ROSL_T_1] |
GSM6652441 |
dpi7_CUT&Tag_H3R27ac_FB:dnAP-1_replicate2 [ROSL_T_2] |
GSM6652442 |
dpi7_CUT&Tag_H3R27ac_CTRL_replicate1 [WT_1] |
GSM6652443 |
dpi7_CUT&Tag_H3R27ac_CTRL_replicate2 [WT_2] |
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This SubSeries is part of SuperSeries: |
GSE215988 |
Single-cell chromatin landscape of non-myocytes reveals AP-1 as a key transcriptional regulator contributing to highly cooperative networks among non-myocyte subtypes during zebrafish heart regeneration |
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Relations |
BioProject |
PRJNA891666 |