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Series GSE216226 Query DataSets for GSE216226
Status Public on Oct 01, 2023
Title TGF-β regulated RNA-binding proteins control endothelial function and mesenchymal transition in cardiac endothelial cells [RIP-seq]
Organism Mus musculus
Experiment type Other
Summary Endothelial to mesenchymal transition (EndoMT) plays a key role in heart development, but is also implicated in cardiovascular diseases in postnatal life. While the roles of TGF-β as inducer of EndoMT on the transcriptional level are well characterised, its post-transcriptional regulatory mechanisms remain largely unknown. Here, we identified global changes in the endothelial mRNA bound proteome upon TGF-β stimulation using RNA interactome capture. Characterisation of TGF-β regulated RNA binding proteins (RBPs) revealed heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1) and Cold Shock Domain Containing E1 (Csde1) as key regulators of endothelial function and EndoMT. We profiled TGF-β driven changes in the RNA binding patterns of hnRNP H1 and Csde1 and found that they dynamically bind and regulate specific subsets of functionally connected RNAs related to mesenchymal activation upon TGF-β stimulation. Together, we show that RBPs play a key role in EndoMT and that the RBPs hnRNP H1 and Csde1 maintain endothelial cell function and counteract mesenchymal activation.
 
Overall design RIP-Seq, input vs Csde1 and hnRNP H1, non-stimulated vs TGF-β (24h, 10 ng/ml)
Web link https://pubmed.ncbi.nlm.nih.gov/37650327/
 
Contributor(s) Wardman R, Keles M, Heineke J
Citation(s) 37650327
BioProject PRJNA889702
Submission date Oct 20, 2022
Last update date Jan 02, 2024
Contact name Joerg Heineke
E-mail(s) joerg.heineke@medma.uni-heidelberg.de
Organization name Medical Faculty of Mannheim Heidelberg University
Department Cardiovascular Physiology
Street address Ludolf-Krehlstr. 7-11 Tridomus B
City Mannheim
ZIP/Postal code 68167
Country Germany
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (14)
GSM6663207 Input_NS
GSM6663208 Input_TGF
GSM6663209 hnRNP_NS_1
This SubSeries is part of SuperSeries:
GSE216228 TGF-β regulated RNA-binding proteins control endothelial function and mesenchymal transition in cardiac endothelial cells

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE216226_Csde1_RIP.xlsx 1.8 Mb (ftp)(http) XLSX
GSE216226_RAW.tar 770.3 Mb (http)(custom) TAR (of BW)
GSE216226_hnRNP_H1_RIP.xlsx 803.7 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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