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Series GSE216263 Query DataSets for GSE216263
Status Public on Apr 15, 2024
Title Transcriptome changes in cardiomyocytes isolated from a rat model of progressive heart failure
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Heart failure is among the leading causes of death globally. Ventricular failure progresses through a hypertrophic compensatory phase followed by failure of the ventricle function through rapid decompensation. In order to unravel right heart specific mechanisms of disease, rat animal models were established that (i) reflect the slowly progressive mode of compensation / decompensation and (ii) allow comparative analyses of left versus right heart failure in the same experimental set up.
Non-restrictive clips around the pulmonary artery (pulmonary artery banding, PAB) or the aorta (aortic banding, AOB) were surgically implanted into weanling rats. Upon animal growth the clips become increasingly constrictive, leading to a compensatory, hypertrophic state at around 6 weeks and heart failure at 21 (PAB) or 24 weeks (AOB). Disease progression was monitored functionally and by sonography.
Differential gene expression analysis was performed for all three treatment groups (sham, AOB, PAB), at both time points (compensation, decompensation) and for both ventricles.
 
Overall design From both phases of AOB and PAB, as well as from matching sham-operated animals, cardiomyocytes (CM) were isolated from the left ventricle and from the right ventricle and total RNA was extracted . Then, total RNAseq was performed after depletion of ribosomal RNAs. A total of 41 animals were included in this study.
 
Contributor(s) Michael K, Axel W, Sebastian W, Liane J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 21, 2022
Last update date Apr 16, 2024
Contact name Axel Weber
Organization name Justus-Liebig-University Giessen
Department Rudolf Buchheim Institute of Pharmakology
Street address Schubertstrasse 81
City Giessen
State/province Hesse
ZIP/Postal code 35392
Country Germany
 
Platforms (1)
GPL25947 Illumina NovaSeq 6000 (Rattus norvegicus)
Samples (82)
GSM6665158 R1_Sham-H_m_LV_#152_rep1
GSM6665159 R2_Sham-H_m_RV_#152_rep1
GSM6665160 R3_Sham-H_m_LV_#159_rep2
Relations
BioProject PRJNA892890
SRA SRP403874

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE216263_RAW.tar 20.3 Gb (http)(custom) TAR (of BIGWIG)
GSE216263_cardiac_myocytes_DEseq2_Output.xlsx 102.4 Mb (ftp)(http) XLSX
GSE216263_cardiac_myocytes_reads.csv.gz 18.8 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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