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| Status |
Public on Nov 20, 2023 |
| Title |
ChIP-seq analysis of the Sordaria macrospora wild type and the developmental mutant delta-asf1 [ChIP-seq] |
| Organism |
Sordaria macrospora |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Genome-wide distribtuion of histone H3 and H3K27me3 (histone H3 tri-methylated at lysine 27) was analyzed in the wild type and the sterile mutant asf1. The asf1 gene encodes a conserved histone chaperone, and preliminary experimentes indicated changes in histone modifications in the mutant that were analyzed on a genome-wide basis in these experiments.
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| Overall design |
The analysis was carried out with mycelia from the wild type and the delta-asf1 mutant. Two biologically independent replicates were obtained for each strain and antibody (against H3 or H3K27me3).
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| Web link |
https://doi.org/10.1128/mbio.02896-23
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| Contributor(s) |
Breuer J, Nowrousian M |
| Citation(s) |
38112417 |
| BioProject |
PRJNA744349 |
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| Submission date |
Oct 26, 2022 |
| Last update date |
Dec 20, 2023 |
| Contact name |
Minou Nowrousian |
| E-mail(s) |
minou.nowrousian@rub.de
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| Phone |
+49 234 3224588
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| Organization name |
Ruhr-University Bochum
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| Department |
Molecular and Cellular Botany
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| Street address |
Universitaetsstr. 150
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| City |
Bochum |
| ZIP/Postal code |
44780 |
| Country |
Germany |
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| Platforms (1) |
| GPL32782 |
Illumina NovaSeq 6000 (Sordaria macrospora) |
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| Samples (16)
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| GSM6684727 |
wild type, histone H3, replicate 1 |
| GSM6684729 |
wild type, histone H3, replicate 2 |
| GSM6684732 |
wild type, H3K27me3, replicate 2 |
| GSM6684735 |
delta-asf1, H3K27me3, replicate 2 |
| GSM6684737 |
delta-asf1, histone H3, replicate 2 |
| GSM6684740 |
delta-asf1, input control for C24 |
| GSM6684743 |
delta-asf1, input control for C25 |
| GSM6684745 |
wild type, input control for C26 |
| GSM6684748 |
wild type, input control for C27 |
| GSM6684751 |
wild type, input control for C28 |
| GSM6684754 |
wild type, input control for C29 |
| GSM6684756 |
delta-asf1, input control for C30 |
| GSM6684759 |
delta-asf1, input control for C31 |
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| This SubSeries is part of SuperSeries: |
| GSE216634 |
Sordaria macrospora wild type and developmental mutant delta-asf1 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE216629_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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