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| Status |
Public on Oct 03, 2023 |
| Title |
Spatial extracellular vesicle microRNA profiles in ovarian cancer |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We newly invented the EV sheet, which is a unique nanofiber paper to capture extracellular vesicles from around 10 uL of bio-fluids. In this study, we investigated miRNA profiles of tumor-surface ascites, serum, and urine using the EV sheet.
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| Overall design |
We attached the EV sheet to the moistened organs to collect a tiny amount of ascites. Moreover, the EV sheet was soaked in a tiny amount of ascites, serum, and urine. Then, total RNA was extracted from the EV sheet, and small RNA libraries were prepared. Finally, a single-end sequence was performed. Similarly, tissue miRNA profiles were obtained.
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| Contributor(s) |
Yokoi A, Yoshida K, Yasui T, Kitagawa M |
| Citation(s) |
37938557 |
| Submission date |
Oct 27, 2022 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Kosuke Yoshida |
| E-mail(s) |
yoshida.kousuke@med.nagoya-u.ac.jp
|
| Organization name |
Nagoya University
|
| Street address |
65 Tsuruma-cho
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
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| Platforms (2) |
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| Samples (138)
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| Relations |
| BioProject |
PRJNA895081 |
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