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Series GSE217070 Query DataSets for GSE217070
Status Public on Nov 05, 2022
Title the natural product erianin induces melanoma apoptosis by targeting GSK3α-NF-κB pathway
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Erianin has been reported to play key roles in suppressing a wide variety of tumors. Howbeit, the mechanism underlying the erianin in anti-carcinogenesis remains to be fully addressed. Half-maximal inhibitory concentration (IC50) was detected to determine the influence of erianin on melanoma in vitro. Subcutaneous xenografts were established to observe the effect of erianin on the proliferation of melanoma cells in vivo. Transcriptome screening was performed to identify the meaningful target. Molecular docking and western blot were performed to verified the GSK3α-NF-κB cascade reaction. We reported the finding of Erianin as an efficient inhibitor of melanoma. Erianin selectively inhibited the growth of melanoma cells, rather than normal human melanocyte cell line. Specifically, erianin downregulated the cellular GSK3α-NF-κB pathway to induce melanoma cell apoptosis. Collectively, we initially proved that erianin inhibited melanoma effectively, which was caused by inactivation of GSK3α-NF-κB signaling inducing melanoma cells apoptosis. Thus, the study implies that erianin provides a novel therapeutic strategy for melanoma therapy.
Overall design RNA-seq for two melanoma cell lines (A375 and MEL-290)
Contributor(s) Zuo S, Li L, Li R, Lu L
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Submission date Nov 02, 2022
Last update date Nov 05, 2022
Contact name Sipeng Zuo
Organization name Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine
Street address No. 12, Lane 833, Zhizaoju Road, Huangpu District
City Shanghai
State/province Shanghai
ZIP/Postal code 200023
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM6704672 A375 CONTROL-1
GSM6704673 A375 CONTROL-2
GSM6704674 A375 ERIANIN-1
BioProject PRJNA896861

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Supplementary file Size Download File type/resource
GSE217070_290_All_Differentially_Expressed_Genes.xlsx 2.2 Mb (ftp)(http) XLSX
GSE217070_A375_All_Differentially_Expressed_Genes.xlsx 2.3 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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