|
| Status |
Public on Nov 05, 2022 |
| Title |
the natural product erianin induces melanoma apoptosis by targeting GSK3α-NF-κB pathway |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Erianin has been reported to play key roles in suppressing a wide variety of tumors. Howbeit, the mechanism underlying the erianin in anti-carcinogenesis remains to be fully addressed. Half-maximal inhibitory concentration (IC50) was detected to determine the influence of erianin on melanoma in vitro. Subcutaneous xenografts were established to observe the effect of erianin on the proliferation of melanoma cells in vivo. Transcriptome screening was performed to identify the meaningful target. Molecular docking and western blot were performed to verified the GSK3α-NF-κB cascade reaction. We reported the finding of Erianin as an efficient inhibitor of melanoma. Erianin selectively inhibited the growth of melanoma cells, rather than normal human melanocyte cell line. Specifically, erianin downregulated the cellular GSK3α-NF-κB pathway to induce melanoma cell apoptosis. Collectively, we initially proved that erianin inhibited melanoma effectively, which was caused by inactivation of GSK3α-NF-κB signaling inducing melanoma cells apoptosis. Thus, the study implies that erianin provides a novel therapeutic strategy for melanoma therapy.
|
| |
|
| Overall design |
RNA-seq for two melanoma cell lines (A375 and MEL-290)
|
| |
|
| Contributor(s) |
Zuo S, Li L, Li R, Lu L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 02, 2022 |
| Last update date |
Nov 05, 2022 |
| Contact name |
Sipeng Zuo |
| E-mail(s) |
Zsp2019@sjtu.edu.cn
|
| Organization name |
Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine
|
| Street address |
No. 12, Lane 833, Zhizaoju Road, Huangpu District
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200023 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA896861 |
Less...
More...