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| Status |
Public on Dec 05, 2022 |
| Title |
Real-time quantitative PCR analysis of microRNAs expressed in adult mouse ventricular-subventricular zone cell populations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
Total RNA was isolated from GFAP::GFP+CD133+EGFR-CD24- (quiescent neural stem cells, qNSCs), GFAP::GFP+CD133+EGFR+CD24- (activated neural stem cells, aNSCs) and GFAP::GFP+CD133- EGFR+CD24- (transit amplifying cells, TACs) cells from the adult mouse ventricular-subventricular zone (V-SVZ) (GFAP::GFP mice, Jackson Mice Stock number 003257).
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| Overall design |
qPCR gene expression profiling. 8 total samples consisting of three biological replicates of qNSCs and aNSCs, and two biological replicates of TACs
miRNA expression profiling by RT-PCR
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| |
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| Contributor(s) |
Doetsch F, Favaloro F, DeLeo AM |
| Citation(s) |
36476846 |
| Submission date |
Nov 07, 2022 |
| Last update date |
Mar 06, 2023 |
| Contact name |
Fiona Doetsch |
| E-mail(s) |
fiona.doetsch@unibas.ch
|
| Organization name |
University of Basel
|
| Department |
Biozentrum
|
| Street address |
Spitalstrasse 41
|
| City |
Basel |
| ZIP/Postal code |
4056 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL17017 |
TaqMan Rodent microRNA A array v2.0 |
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| Samples (8)
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| GSM6718627 |
adult V-SVZ qNSCs biological replicate 1 |
| GSM6718628 |
adult V-SVZ qNSCs biological replicate 2 |
| GSM6718629 |
adult V-SVZ qNSCs biological replicate 3 |
| GSM6718630 |
adult V-SVZ aNSCs biological replicate 1 |
| GSM6718631 |
adult V-SVZ aNSCs biological replicate 2 |
| GSM6718632 |
adult V-SVZ aNSCs biological replicate 3 |
| GSM6718633 |
adult V-SVZ TACs biological replicate 1 |
| GSM6718634 |
adult V-SVZ TACs biological replicate 2 |
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| Relations |
| BioProject |
PRJNA898997 |
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