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Series GSE21798 Query DataSets for GSE21798
Status Public on Jun 01, 2010
Title Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster (miRNA Agilent Part)
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary The generation of myelinating cells in the central nervous system (CNS) requires the initiation of specific gene-expression programs in oligodendrocytes. We reasoned that miRNAs could play an important role in this process by regulating critical developmental genes. Microarray profiling of cultured oligodendrocytes identifies the miR-17~92 family of miRNA cluster as highly enriched miRNAs in oligodendrocytes. We specifically deleted the miR-17~92 cluster in oligodendrocytes using the 2´3´-cyclic nucleotide 3´-phosphodiesterase (CNP)-Cre mice. Absence of miR-17~92 leads to a reduction of oligodendrocyte number in vivo and we find that the expression of these miRNAs in primary cultures of oligodendrocytes promotes cell proliferation by influencing Akt signalling. Together, these results suggest that the miRNA pathway is essential in determining oligodendroglial cell number and that the miR-17~92 cluster is crucial in this process.
Overall design MicroRNA microarray profiling was used for the identification of miRNAs enriched in the oligodendroglial lineage. Total RNA lysates from immature (one and two days in oligodendrocyte precursor cell (OPC) culture -d0 and d1) and mature oligodendrocytes (six days in culture - d6) were analysed regarding their miRNA levels. Three independent samples for each time point were used (two samples for day 1).
Contributor(s) Budde H, Schmitt S, Fitzner D, Opitz L, Salinas-Riester G, Simons M
Citation(s) 20504959
Submission date May 12, 2010
Last update date Jul 14, 2015
Contact name Gabriela Salinas
Organization name Universitaetsmedizin Goettingen
Department Department of Pathology
Lab NGS Integrative Genomics
Street address Kreuzbergring 57
City Goettingen
State/province Lower-Saxony
ZIP/Postal code 37075
Country Germany
Platforms (1)
GPL10384 Agilent-021828 Unrestricted Mouse miRNA Microarray (V2) (Feature Number version)
Samples (8)
GSM542910 Immature oligodendrocytes d0_Rep1
GSM542911 Immature oligodendrocytes d0_Rep2
GSM542912 Immature oligodendrocytes d0_Rep3
This SubSeries is part of SuperSeries:
GSE21801 Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster
BioProject PRJNA129341

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21798_RAW.tar 13.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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