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Status |
Public on Jun 01, 2010 |
Title |
Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster (miRNA Agilent Part) |
Organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by array
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Summary |
The generation of myelinating cells in the central nervous system (CNS) requires the initiation of specific gene-expression programs in oligodendrocytes. We reasoned that miRNAs could play an important role in this process by regulating critical developmental genes. Microarray profiling of cultured oligodendrocytes identifies the miR-17~92 family of miRNA cluster as highly enriched miRNAs in oligodendrocytes. We specifically deleted the miR-17~92 cluster in oligodendrocytes using the 2´3´-cyclic nucleotide 3´-phosphodiesterase (CNP)-Cre mice. Absence of miR-17~92 leads to a reduction of oligodendrocyte number in vivo and we find that the expression of these miRNAs in primary cultures of oligodendrocytes promotes cell proliferation by influencing Akt signalling. Together, these results suggest that the miRNA pathway is essential in determining oligodendroglial cell number and that the miR-17~92 cluster is crucial in this process.
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Overall design |
MicroRNA microarray profiling was used for the identification of miRNAs enriched in the oligodendroglial lineage. Total RNA lysates from immature (one and two days in oligodendrocyte precursor cell (OPC) culture -d0 and d1) and mature oligodendrocytes (six days in culture - d6) were analysed regarding their miRNA levels. Three independent samples for each time point were used (two samples for day 1).
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Contributor(s) |
Budde H, Schmitt S, Fitzner D, Opitz L, Salinas-Riester G, Simons M |
Citation(s) |
20504959 |
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Submission date |
May 12, 2010 |
Last update date |
Jul 14, 2015 |
Contact name |
Gabriela Salinas |
E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
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Organization name |
Universitaetsmedizin Goettingen
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Department |
Department of Pathology
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Lab |
NGS Integrative Genomics
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Street address |
Kreuzbergring 57
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City |
Goettingen |
State/province |
Lower-Saxony |
ZIP/Postal code |
37075 |
Country |
Germany |
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Platforms (1) |
GPL10384 |
Agilent-021828 Unrestricted Mouse miRNA Microarray (V2) (Feature Number version) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE21801 |
Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster |
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Relations |
BioProject |
PRJNA129341 |