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Series GSE218212 Query DataSets for GSE218212
Status Public on Apr 30, 2023
Title RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding [scRNA-seq_ScaleUp_Scipio]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Progress in sample preparation for scRNA-seq is reported based on RevGel-seq, a reversible-hydrogel technology. Barcode bead-cell tandems stabilized by a chemical linker are dispersed in the hydrogel in the liquid state. Upon gelation the tandems are immobilized, cell lysis is triggered by detergent diffusion, and RNA molecules are captured on the adjacent barcode beads. After reverse transcription and preparation for cDNA sequencing, bioinformatic analysis reveals performance quality comparable to microfluidic-based technologies.
 
Overall design Benchmarking experiments to assess the performances obtained using RevGel-seq (scRNA preparation).
 
Contributor(s) Eckert H, Guérin F, Poncin R, Majello S, Cathaly L, Lipin S, André B, Edelstein S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 17, 2022
Last update date May 02, 2023
Contact name Stuart EDELSTEIN
Organization name Scipio bioscience
Street address 29 rue du Faubourg Saint Jacques, Pépinière Paris Santé Cochin
City Paris
State/province Île-de-France
ZIP/Postal code 75014
Country France
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (2)
GSM6736637 human PBMC control
GSM6736638 human PBMC 3x loading
Relations
BioProject PRJNA902750

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE218212_RAW.tar 3.5 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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