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| Status |
Public on Oct 18, 2023 |
| Title |
Histone retention preserves epigenetic marks during heat stress-induced transcriptional memory |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Plants often experience recurrent stressful events, for example during heat waves. They can adapt to such recurrent heat stress (HS), allowing subsequent survival of more severe HS conditions. At certain genes HS induces sustained expression for several days beyond the actual HS. This transcriptional memory is associated with hyper-methylation of histone H3 lysine 4 (H3K4me3), however, how this is maintained for extended periods of time is unclear. Here, we determined histone turnover by measuring chromatin association of a HS-induced histone H3.3. Genome-wide Histone turnover was not homogenous, in particular, H3.3 was retained longer at HS memory genes compared to HS-induced non-memory genes during the memory phase. While low nucleosome turnover retained H3K4 methylation, its loss did not affect turnover, suggesting that low nucleosome turnover sustains H3K4 methylation (and not vice versa). Together, our results unveil the modulation of histone turnover as a mechanism to retain environmentally-mediated epigenetic modifications.
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| Overall design |
ChIP-seq to estimate histone turnover using pulse-labelled histone H3.3.
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| Contributor(s) |
Pratx L, Kappel C, Bäurle I |
| Citation(s) |
37937667 |
| |
| Submission date |
Nov 17, 2022 |
| Last update date |
Jan 02, 2024 |
| Contact name |
Isabel Bäurle |
| E-mail(s) |
isabel.baeurle@uni-potsdam.de
|
| Phone |
+49 331 9772647
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| Organization name |
Universität Potsdam
|
| Department |
Institut für Biochemie und Biologie
|
| Street address |
Karl-Liebknecht-Str. 24-25
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| City |
Potsdam |
| ZIP/Postal code |
14476 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA902769 |
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