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| Status |
Public on Dec 01, 2023 |
| Title |
Effect of long-term exposure of platinum nanopariticles on gene expression of Pseudomonas aeruginosa PAO1 |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Previous synthesized Pt NPs were selected to evaluate the influences on bacterial resistance, and a typical pathogenic microbe P. aeruginosa was chosen as model bacteria.
After 60-day PtNPs exposure, we found under 12.5 μg/mL of platinum nanoparticles (PtNPs) exposure for ~7200 generations, the IC50 of evolved Pseudomonas aeruginosa PAO1 to imipenem (IPM) and ciprofloxacin (CIP) reduced 77.0% and 87.8%, respectively. Interestingly, long-term of PtNPs exposure arose the bacterial susceptibility on antibiotics. We then performed gene expression profiling analysis using data obtained from RNA-seq.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for wild-type strains (WT), non-exposed but evolved PAO1 (CK), and 60-day PtNPs exposed and evolved PAO1 (ES)
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| Contributor(s) |
Lian Y, Wang M |
| Citation missing |
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| Submission date |
Nov 21, 2022 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Yulu Lian |
| E-mail(s) |
20020160048@pop.zjgsu.edu.cn
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| Phone |
+8619858172692
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| Organization name |
Zhejiang Gongshang University
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| Street address |
Hangzhou
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| City |
Hangzhou,Zhejiang |
| State/province |
Zhejiang |
| ZIP/Postal code |
310035 |
| Country |
China |
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| Platforms (1) |
| GPL18287 |
Illumina HiSeq 2500 (Pseudomonas aeruginosa) |
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| Samples (9)
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| GSM6744369 |
CK-1, non-exposed but evolved PAO1 |
| GSM6744370 |
CK-2, non-exposed but evolved PAO1 |
| GSM6744371 |
CK-3, non-exposed but evolved PAO1 |
| GSM6744372 |
ES-1, 60-day PtNPs exposed and evolved PAO1 (ES) |
| GSM6744373 |
ES-2,60-day PtNPs exposed and evolved PAO1 (ES) |
| GSM6744374 |
ES-3,60-day PtNPs exposed and evolved PAO1 |
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| Relations |
| BioProject |
PRJNA903796 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE218408_RAW.tar |
670.0 Kb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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