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Series GSE218408 Query DataSets for GSE218408
Status Public on Dec 01, 2023
Title Effect of long-term exposure of platinum nanopariticles on gene expression of Pseudomonas aeruginosa PAO1
Organism Pseudomonas aeruginosa
Experiment type Expression profiling by high throughput sequencing
Summary Previous synthesized Pt NPs were selected to evaluate the influences on bacterial resistance, and a typical pathogenic microbe P. aeruginosa was chosen as model bacteria.
After 60-day PtNPs exposure, we found under 12.5 μg/mL of platinum nanoparticles (PtNPs) exposure for ~7200 generations, the IC50 of evolved Pseudomonas aeruginosa PAO1 to imipenem (IPM) and ciprofloxacin (CIP) reduced 77.0% and 87.8%, respectively. Interestingly, long-term of PtNPs exposure arose the bacterial susceptibility on antibiotics. We then performed gene expression profiling analysis using data obtained from RNA-seq.
Overall design Comparative gene expression profiling analysis of RNA-seq data for wild-type strains (WT), non-exposed but evolved PAO1 (CK), and 60-day PtNPs exposed and evolved PAO1 (ES)
Contributor(s) Lian Y, Wang M
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Submission date Nov 21, 2022
Last update date Dec 01, 2023
Contact name Yulu Lian
Phone +8619858172692
Organization name Zhejiang Gongshang University
Street address Hangzhou
City Hangzhou,Zhejiang
State/province Zhejiang
ZIP/Postal code 310035
Country China
Platforms (1)
GPL18287 Illumina HiSeq 2500 (Pseudomonas aeruginosa)
Samples (9)
GSM6744366 WT-1, wild-type strains
GSM6744367 WT-2, wild-type strains
GSM6744368 WT-3, wild-type strains
BioProject PRJNA903796

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE218408_RAW.tar 670.0 Kb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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