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Series GSE21860 Query DataSets for GSE21860
Status Public on Jul 02, 2010
Title RNA-seq of pancreatic islets isolated from normal female and pregnant female mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During pregnancy, the energy requirements of the fetus impose changes in maternal metabolism. Increasing insulin resistance in the mother maintains nutrient flow to the growing fetus, while prolactin and placental lactogen counterbalance this resistance and prevent maternal hyperglycemia by driving expansion of the maternal population of insulin-producing beta-cells. However, the exact mechanisms by which the lactogenic hormones drive beta-cell expansion remain uncertain. Here we show that serotonin acts downstream of lactogen signaling to drive beta-cell proliferation. Serotonin synthetic enzyme Tph1 and serotonin production increased sharply in beta-cells during pregnancy or after treatment with lactogens in vitro. Inhibition of serotonin synthesis by dietary tryptophan restriction or Tph inhibition blocked beta-cell expansion and induced glucose intolerance in pregnant mice without affecting insulin sensitivity. Expression of the Gq-linked serotonin receptor Htr2b in maternal islets increased during pregnancy and normalized just prior to parturition, while expression of the Gi-linked receptor Htr1d increased at the end of pregnancy and postpartum. Blocking Htr2b signaling in pregnant mice also blocked beta-cell expansion and caused glucose intolerance. These studies reveal an integrated signaling pathway linking beta-cell mass to anticipated insulin need during pregnancy. Modulators of this pathway, including medications and diet, may affect the risk of gestational diabetes.
Overall design Analysis of poly(A)+ RNA from 3 biological replicates of pancreatic islets isolated from normal female and pregnant female mice
Contributor(s) Kim H, Lynn FF, German MS
Citation(s) 20581837
Submission date May 17, 2010
Last update date May 15, 2019
Contact name Hail Kim
Organization name University of California San Francisco
Department Diabetes Center
Lab Michale German's Lab
Street address 513 Parnassus avenue HSW1090
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (6)
GSM543645 Pancreatic islets-nonpregnant-rp1
GSM543646 Pancreatic islets-nonpregnant-rp2
GSM543647 Pancreatic islets-nonpregnant-rp3
SRA SRP002569
BioProject PRJNA127041

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21860.txt.gz 261.5 Kb (ftp)(http) TXT
GSE21860_RAW.tar 1004.0 Mb (http)(custom) TAR (of TXT)
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Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

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