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Series GSE21891 Query DataSets for GSE21891
Status Public on Aug 03, 2015
Title Expression data from insulin-treated human primary fibroblasts and effects of U0126 on insulin-induced gene expression
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We carried out a high throughput analysis of insulin-induced kinase signaling pathways in primary fibroblasts from 35 unrelated individuals. We found that extensive individual variation exists in induction of various signaling pathways. ERK signaling displayed the greatest variation, which led to extensive variation in expression of downstream target genes.
Our results suggest that phenotypic variation in kinase signaling mediates variation in downstream processes of insulin response. Future study of such phenotypic variation is important to linking genetic variants to individual susceptibility to complex diseases such as diabetes.
Overall design Passage-matched primary fibroblasts from 35 unrelated newborns (foreskin) were treated with 100 nM insulin. We measured gene expression levels in each of 35 individuals at baseline, and 1 hour and 6 hours after insulin treatment using expression arrays.
In order to examine effects of ERK inhibition on insulin-induced gene expression, we treated fibroblasts from 4 individuals with DMSO or 10uM of U0126 for 1 hour, followed by insulin treatment for one hour and 6 hours. We then harvested cells and measured gene expression in each sample using expression arrays.
Contributor(s) Wang IX, Cheung VG
Citation(s) 26202599
Submission date May 18, 2010
Last update date Dec 06, 2018
Contact name Isabel Xiaorong Wang
Organization name HHMI/University of Michigan
Department Pediatrics&Genetics
Lab Dr. Vivian G. Cheung Lab
Street address 210 washtenaw ave
City Ann Arbor
State/province Michigan
ZIP/Postal code 48109
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (129)
GSM544288 FB3132_0m_1
GSM544289 FB3132_360m_73
GSM544290 FB3132_60m_37
BioProject PRJNA126905

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21891_LuminexAssayResult.xlsx 29.2 Kb (ftp)(http) XLSX
GSE21891_Microarray_Insulin_responsive_genes_GEO.xlsx 322.9 Kb (ftp)(http) XLSX
GSE21891_RAW.tar 265.2 Mb (http)(custom) TAR (of CEL, CHP)
GSE21891_README_Supplementary_file_descriptions.txt 969 b (ftp)(http) TXT
GSE21891_Table_gene_kinase_corr.xlsx 177.6 Kb (ftp)(http) XLSX
GSE21891_Wangetal_SILAC_ProteinGroups.txt.gz 486.4 Kb (ftp)(http) TXT
GSE21891_Wangetal_SILAC_allPeptides.txt.gz 28.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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