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Series GSE219052 Query DataSets for GSE219052
Status Public on Nov 28, 2023
Title Influence of Sox4 on chromatin accessibility of adult hepatocytes [EV and Sox4]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Recent studies have demonstrated that hepatocytes can be reprogrammed into biliary epithelial cell-like cells. An earlier work in our laboratory nominated Sox4 as an initiation factor of this reprogramming event To investigate the effect of Sox4 on the change of chromatin landscape of hepatocytes, we performed ATAC-seq of exogenously Sox4-expressed hepatocytes.
Overall design Mice were injected with adeno-associated viruses (AAVs) packaged with mouse Sox4 (AAV8-TBG-HA-Sox4-P2A-Cre) or empty virus (AAV8-TBG-Cre), and hepatocytes were harvested 4 days post injection by a standard 2-step liberase digestion method followed by a Percoll enrichment step.
Contributor(s) Katsuda T, Sussman J, Stanger BZ
Citation(s) 38409161
Submission date Nov 30, 2022
Last update date Mar 11, 2024
Contact name Takeshi Katsuda
Phone +1-215-746-5559
Organization name University of Pennsylvania
Department Perelman School of Medicine
Lab Gastroenterology Division
Street address 421 Curie Blvd, BRB II/III Rm531
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM6766771 EV hepatocytes, replicate1
GSM6766772 EV hepatocytes, replicate2
GSM6766773 EV hepatocytes, replicate3
This SubSeries is part of SuperSeries:
GSE221225 Sox4 in adult hepatocytes
BioProject PRJNA906935

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 1.1 Gb (ftp)(http) BW 1.5 Gb (ftp)(http) BW
GSE219052_RAW.tar 3.5 Gb (http)(custom) TAR (of BW, XLS)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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