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Series GSE220040 Query DataSets for GSE220040
Status Public on Apr 12, 2023
Title CMTR1 promotes colorectal cancer cell growth and immune evasion by transcriptionally regulating STAT3
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary CMTR1, also called IFN-stimulated gene 95 kDa protein (ISG95), is elevated by viral infection in a variety of cells. However, the function of CMTR1 in colorectal cancer (CRC), especially its role in tumorigenesis and immune regulation, remains unclear. Here, we first identified CMTR1 as a novel oncogene in colorectal cancer. Based on The Cancer Genome Atlas (TCGA) database exploration and human tissue microarray (TMA) analysis, we found that CMTR1 expression was markedly higher in colorectal cancer tissues compared to that in adjacent normal tissues. High CMTR1 was correlated with poor prognosis of colorectal cancer patients. Knockdown (KD) of CMTR1 significantly suppressed cell proliferation and tumorigenicity both in vitro and in vivo, whereas overexpression of CMTR1 exhibited the opposite effects. KEGG analysis revealed that the JAK/STAT signaling pathway was differentially enriched in colorectal cancer cells with CMTR1 KD. Mechanistically, repression of CMTR1 influenced RNAPII recruitment to the TSS and repression of STAT3 expression and activation. Furthermore, PD1 blockade immunotherapy was prominently enhanced with CMTR1 KD by increased infiltration of CD8+ T cells into tumor microenvironment. Overall, it appears that CMTR1 plays a key role in regulating tumor cell proliferation and antitumor immunity.
Overall design RNA-seq performed in this study was designed to understand the molecular mechanisms underlying CMTR1 in gene transcription regulation.
Contributor(s) You A, Ding N, Ye F
Citation(s) 37024465
Submission date Dec 03, 2022
Last update date Apr 13, 2023
Contact name you a bin
Phone 18811180581
Organization name the First Affiliated Hospital of Xiamen University
Street address 55 Zhenhai Road, Siming District, Xiamen
City Xiamen
State/province Fujian
ZIP/Postal code 361003
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (2)
GSM6783836 RNA-Seq-siNC-RKO
GSM6783837 RNA-Seq-siCMTR1-RKO
BioProject PRJNA908097

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE220040_RAW.tar 172.3 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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