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| Status |
Public on Nov 27, 2023 |
| Title |
Suclg2 maintains the tolerogenic function of regulatory dendritic cells by suppressing Lactb succinylation [si-nc/si-Suclg2] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: mRNAs sequencing of the gene expression differences in diffDC transfected with si-nc or si-Suclg2, the goals of this study are to investigate the biological effect of the metabolic enzyme succinate-CoA ligase subunit beta Suclg2 in regulating regulatory DC differentiation and function.
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| Overall design |
Bone marrow cells from C57/BL6 mice were cultured with GM-CSF and IL-4 for 6 days and stimulated with LPS for 24 hours to generate bone-marrow derived DC (BMDC). CD11c+ BMDC were isolated by anti-mouse CD11c magbeads to generate maDC. maDC were then cocultured with spleen stroma cells for 9 days to generate diffDC. And then diffDCs were transfected with si-Suclg2 or si-nc. diffDCs transfected with si-Suclg2 or si-nc were subjected to RNA seq.
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| Contributor(s) |
Zhang X, Cao X |
| Citation(s) |
37216870 |
| |
| Submission date |
Dec 04, 2022 |
| Last update date |
Nov 27, 2023 |
| Contact name |
xiaomin zhang |
| E-mail(s) |
xiaoxiao0666@126.com
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| Phone |
18217743328
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| Organization name |
National Key Laboratory of Medical Immunology & Institute of Immunology, Naval Military Medical University
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| Street address |
800 Xiang Yin Road
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| City |
shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA908261 |
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