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| Status |
Public on Dec 07, 2022 |
| Title |
Cytokines in the ApoE-/- mice atherosclerotic plaque |
| Organism |
Mus musculus |
| Experiment type |
Protein profiling by protein array
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| Summary |
To identify the dynamic changes of cytokines in the ApoE-/- mice atherosclerotic plaque, heart, and serum upon β-adrenergic stimulation (isoproterenol, 5 mg/kg body weight) at 0.5hrs, 1hrs, 3hrs and 24hrs using mouse cytokine antibody arrays.
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| Overall design |
45 samples. Male ApoE knockout mice (8 weeks of age, C57BL/6J background) fed a high fat diet containing 40 kcal% fat and 1.25% cholesterol (D12108C, Reaserch Diets, USA) for 2 weeks. Carotid collar placement was performed 2 weeks later to induce carotid artery plaque. After carotid collar placement and high-fat feeding for 3 months, mice were subcutaneously injected with a single dose of ISO (Sigma-Aldrich, St. Louis, MO, USA; 5 mg/kg body weight, as determined effective by dose-response preliminary experiments) or saline (10 mL/kg body weight).There are 9 groups: vehicle group, mice were subcutaneous injected with a single dose of saline (10 ml/kg body weight), ISO groups, mice were subcutaneous injected with a single dose of isoproterenol (5 mg/kg body weight) after 0.5hrs, 1hrs, 3hrs and 24hrs (vessel, heart and plasma groups by different pionts)
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| Contributor(s) |
Chen X, Xiao H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 06, 2022 |
| Last update date |
Dec 07, 2022 |
| Contact name |
Xianda Chen |
| E-mail(s) |
chenxd@bjmu.edu.cn
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| Organization name |
Peking University Third Hospital
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| Department |
Institute of Vascular Medicine
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| Street address |
No. 49, Huayuan Bei Road, Haidian District
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| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (1) |
| GPL32918 |
Bio-plex pro mouse chemokine Panel 31-Plex |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA909254 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220225_raw_intensities.txt.gz |
8.1 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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