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Status |
Public on Dec 07, 2022 |
Title |
Cytokines in the ApoE-/- mice atherosclerotic plaque |
Organism |
Mus musculus |
Experiment type |
Protein profiling by protein array
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Summary |
To identify the dynamic changes of cytokines in the ApoE-/- mice atherosclerotic plaque, heart, and serum upon β-adrenergic stimulation (isoproterenol, 5 mg/kg body weight) at 0.5hrs, 1hrs, 3hrs and 24hrs using mouse cytokine antibody arrays.
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Overall design |
45 samples. Male ApoE knockout mice (8 weeks of age, C57BL/6J background) fed a high fat diet containing 40 kcal% fat and 1.25% cholesterol (D12108C, Reaserch Diets, USA) for 2 weeks. Carotid collar placement was performed 2 weeks later to induce carotid artery plaque. After carotid collar placement and high-fat feeding for 3 months, mice were subcutaneously injected with a single dose of ISO (Sigma-Aldrich, St. Louis, MO, USA; 5 mg/kg body weight, as determined effective by dose-response preliminary experiments) or saline (10 mL/kg body weight).There are 9 groups: vehicle group, mice were subcutaneous injected with a single dose of saline (10 ml/kg body weight), ISO groups, mice were subcutaneous injected with a single dose of isoproterenol (5 mg/kg body weight) after 0.5hrs, 1hrs, 3hrs and 24hrs (vessel, heart and plasma groups by different pionts)
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Contributor(s) |
Chen X, Xiao H |
Citation missing |
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Submission date |
Dec 06, 2022 |
Last update date |
Dec 07, 2022 |
Contact name |
Xianda Chen |
E-mail(s) |
chenxd@bjmu.edu.cn
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Organization name |
Peking University Third Hospital
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Department |
Institute of Vascular Medicine
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Street address |
No. 49, Huayuan Bei Road, Haidian District
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City |
Beijing |
ZIP/Postal code |
100191 |
Country |
China |
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Platforms (1) |
GPL32918 |
Bio-plex pro mouse chemokine Panel 31-Plex |
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Samples (45)
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Relations |
BioProject |
PRJNA909254 |
Supplementary file |
Size |
Download |
File type/resource |
GSE220225_raw_intensities.txt.gz |
8.1 Kb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
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