 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 07, 2022 |
| Title |
Age-associated downregulation of glutamate and GABA neurotransmission-related gene expression in the rostral ventrolateral medulla of male Fischer 344 rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
The rostral ventrolateral medulla (RVLM), a part of the medullary reticular formation, plays a major role in several physiological responses, including cardiovascular and sympathetic nervous system functions. Although aging causes disturbances in the responses of these physiological systems, RVLM involvement in these age-related changes is not clear. Previous work using high-throughput gene expression analysis of the RVLM in aged animals suggested that chemical neurotransmission-related genes might be downregulated with advancing age. Since the RVLM function involves a balance of inhibitory and excitatory inputs, which is largely mediated by gamma-aminobutyric acid (GABA) and excitatory amino acid (EAA) neurotransmission, we hypothesized that aging is associated with altered excitatory and/or inhibitory neurotransmission-related gene expression in the RVLM. To test this hypothesis, we micropunched an RVLM-containing area from young (3–5 months), middle-aged (12–14 months), and aged (22–26 months) Fischer 344 male rats. RNA purified from these micropunches was analyzed using GABA and Glutamate RT2 Profiler PCR arrays (n= 8–10). In addition, the expression of selected genes was validated at the RNA level using TaqMan® based- qPCR and at the protein level using western blotting. All the genes that displayed significant differential expression (1.5-fold, p < .05, FDR < .05) were identified to be downregulated in the RVLM of aged and middle-aged rats compared to young rats. Among the downregulated genes, the percentage of glutamate neurotransmission-related genes was higher than GABA neurotransmission-related genes. Solute carrier family 1 member 6 (Slc1a6) gene showed the highest fold downregulation at the RNA level in the RVLM of aged compared to young rats, and its protein product, Excitatory amino acid transporter 4 (EAAT4), showed a downregulatory trend in the RVLM of aged and middle-aged rats. These results suggest that molecular constituents of both GABA and glutamate neurotransmission might be altered in the RVLM of aged and middle-aged rats, and the changes in glutamate neurotransmission might be more prominent. Investigating age-associated anatomical and functional changes in RVLM GABA and glutamate neurotransmission might provide a foundation for understanding the effects of aging on physiological function.
|
| |
|
| Overall design |
Rostral ventrolateral medulla (RVLM) containing lower brainstem area was micropunched from young (3–5 months, 304 ± 7 g, n=8), middle-aged (12–14 months, 425 ± 8 g, n=8), and aged (22–26 months, 432 ± 11 g, n=8) male Fischer 344 rats. RNA collected from these tissue punches was analyzed using Rat GABA & Glutamate RT2 Profiler PCR arrays (Qiagen, Inc., GeneGlobe ID PARN-152Z).
|
| |
|
| Contributor(s) |
Balivada S, Tapia GP, Pawar HN, Khan AM, Kenney MJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AG041948 |
Sympathetic Neural Regulation and Aging: Medullary Mechanisms and Strategies |
KANSAS STATE UNIVERSITY |
Michael J Kenney |
| SC1 GM127251 |
Interrogating brain circuits that control feeding caused by fasting or by craving |
UNIVERSITY OF TEXAS EL PASO |
ARSHAD M. KHAN |
|
| Submission date |
Dec 07, 2022 |
| Last update date |
Dec 07, 2022 |
| Contact name |
Sivasai Balivada |
| E-mail(s) |
sbalivada@utep.edu
|
| Phone |
7854777070
|
| Organization name |
University of Texas at El Paso
|
| Street address |
4th floor, Bioscience research building
|
| City |
El Paso |
| State/province |
Texas |
| ZIP/Postal code |
79968 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL32921 |
Qiagen Rat GABA & Glutamate RT2 Profiler PCR assay |
|
| Samples (26)
|
| GSM6797916 |
Young RVLM, set1, biological replicate 1 |
| GSM6797917 |
Young RVLM, set1, biological replicate 2 |
| GSM6797918 |
Young RVLM, set1, biological replicate 3 |
| GSM6797919 |
Young RVLM, set1, biological replicate 4 |
| GSM6797920 |
Young RVLM, set1, biological replicate 5 |
| GSM6797921 |
Young RVLM, set2, biological replicate 6 |
| GSM6797922 |
Young RVLM, set2, biological replicate 7 |
| GSM6797923 |
Young RVLM, set2, biological replicate 8 |
| GSM6797924 |
Middle-aged RVLM, set1, biological replicate 1 |
| GSM6797925 |
Middle-aged RVLM, set1, biological replicate 2 |
| GSM6797926 |
Middle-aged RVLM, set1, biological replicate 3 |
| GSM6797927 |
Middle-aged RVLM, set1, biological replicate 4 |
| GSM6797928 |
Middle-aged RVLM, set1, biological replicate 5 |
| GSM6797929 |
Middle-aged RVLM, set2, biological replicate 6 |
| GSM6797930 |
Middle-aged RVLM, set2, biological replicate 7 |
| GSM6797931 |
Middle-aged RVLM, set2, biological replicate 8 |
| GSM6797932 |
Aged RVLM, set1, biological replicate 1 |
| GSM6797933 |
Aged RVLM, set1, biological replicate 2 |
| GSM6797934 |
Aged RVLM, set1, biological replicate 3 |
| GSM6797935 |
Aged RVLM, set1, biological replicate 4 |
| GSM6797936 |
Aged RVLM, set1, biological replicate 5 |
| GSM6797937 |
Aged RVLM, set2, biological replicate 6 |
| GSM6797938 |
Aged RVLM, set2, biological replicate 7 |
| GSM6797939 |
Aged RVLM, set2, biological replicate 8 |
| GSM6797940 |
Aged RVLM, set2, biological replicate 9 |
| GSM6797941 |
Aged RVLM, set2, biological replicate 10 |
|
| Relations |
| BioProject |
PRJNA909594 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220296_Fold_change.xlsx |
13.5 Kb |
(ftp)(http) |
XLSX |
| GSE220296_Matrix_non-normalized.xlsx |
40.0 Kb |
(ftp)(http) |
XLSX |
| Processed data included within Sample table |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...