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Series GSE220296 Query DataSets for GSE220296
Status Public on Dec 07, 2022
Title Age-associated downregulation of glutamate and GABA neurotransmission-related gene expression in the rostral ventrolateral medulla of male Fischer 344 rats
Organism Rattus norvegicus
Experiment type Expression profiling by RT-PCR
Summary The rostral ventrolateral medulla (RVLM), a part of the medullary reticular formation, plays a major role in several physiological responses, including cardiovascular and sympathetic nervous system functions. Although aging causes disturbances in the responses of these physiological systems, RVLM involvement in these age-related changes is not clear. Previous work using high-throughput gene expression analysis of the RVLM in aged animals suggested that chemical neurotransmission-related genes might be downregulated with advancing age. Since the RVLM function involves a balance of inhibitory and excitatory inputs, which is largely mediated by gamma-aminobutyric acid (GABA) and excitatory amino acid (EAA) neurotransmission, we hypothesized that aging is associated with altered excitatory and/or inhibitory neurotransmission-related gene expression in the RVLM. To test this hypothesis, we micropunched an RVLM-containing area from young (3–5 months), middle-aged (12–14 months), and aged (22–26 months) Fischer 344 male rats. RNA purified from these micropunches was analyzed using GABA and Glutamate RT2 Profiler PCR arrays (n= 8–10). In addition, the expression of selected genes was validated at the RNA level using TaqMan® based- qPCR and at the protein level using western blotting. All the genes that displayed significant differential expression (1.5-fold, p < .05, FDR < .05) were identified to be downregulated in the RVLM of aged and middle-aged rats compared to young rats. Among the downregulated genes, the percentage of glutamate neurotransmission-related genes was higher than GABA neurotransmission-related genes. Solute carrier family 1 member 6 (Slc1a6) gene showed the highest fold downregulation at the RNA level in the RVLM of aged compared to young rats, and its protein product, Excitatory amino acid transporter 4 (EAAT4), showed a downregulatory trend in the RVLM of aged and middle-aged rats. These results suggest that molecular constituents of both GABA and glutamate neurotransmission might be altered in the RVLM of aged and middle-aged rats, and the changes in glutamate neurotransmission might be more prominent. Investigating age-associated anatomical and functional changes in RVLM GABA and glutamate neurotransmission might provide a foundation for understanding the effects of aging on physiological function.
Overall design Rostral ventrolateral medulla (RVLM) containing lower brainstem area was micropunched from young (3–5 months, 304 ± 7 g, n=8), middle-aged (12–14 months, 425 ± 8 g, n=8), and aged (22–26 months, 432 ± 11 g, n=8) male Fischer 344 rats. RNA collected from these tissue punches was analyzed using Rat GABA & Glutamate RT2 Profiler PCR arrays (Qiagen, Inc., GeneGlobe ID PARN-152Z).
Contributor(s) Balivada S, Tapia GP, Pawar HN, Khan AM, Kenney MJ
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NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AG041948 Sympathetic Neural Regulation and Aging: Medullary Mechanisms and Strategies KANSAS STATE UNIVERSITY Michael J Kenney
SC1 GM127251 Interrogating brain circuits that control feeding caused by fasting or by craving UNIVERSITY OF TEXAS EL PASO ARSHAD M. KHAN
Submission date Dec 07, 2022
Last update date Dec 07, 2022
Contact name Sivasai Balivada
Phone 7854777070
Organization name University of Texas at El Paso
Street address 4th floor, Bioscience research building
City El Paso
State/province Texas
ZIP/Postal code 79968
Country USA
Platforms (1)
GPL32921 Qiagen Rat GABA & Glutamate RT2 Profiler PCR assay
Samples (26)
GSM6797916 Young RVLM, set1, biological replicate 1
GSM6797917 Young RVLM, set1, biological replicate 2
GSM6797918 Young RVLM, set1, biological replicate 3
BioProject PRJNA909594

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE220296_Fold_change.xlsx 13.5 Kb (ftp)(http) XLSX
GSE220296_Matrix_non-normalized.xlsx 40.0 Kb (ftp)(http) XLSX
Processed data included within Sample table
Processed data are available on Series record

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