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Series GSE220303 Query DataSets for GSE220303
Status Public on Sep 23, 2023
Title 3D genome remodeling and homologous pairing during meiotic prophase of mouse oogenesis and spermatogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We comprehensively compared the chromatin structures and transcriptomes in successive substages of female and male mouse meiotic prophase by using sisHi-C and RNA-Seq methods. Interestingly, the transcriptional change happened earlier than chromatin structures reprograming that chromatin structures largely maintained the pre-meiotic condition in leptotene. Also, compartments and TADs gradually disappeared and then slowly recovered in both oocytes and spermatocytes. We characterized the events of homologous chromosomes pairing and found homologues adopted two sex-conserved pairing strategies prior to and after leptotene-to-zygotene transition, which firstly contacted more frequently in LINE enriched compartment B and then switched to SINE enriched compartment A. The sexual difference of transcriptome was most obvious in late meiotic prophase, which reflected in gamete morphology and function differences. We also complemented marker genes for each substage of oocytes and spermatocytes meiotic prophase, and predicted the sex-specific meiotic functional genes, whose mutation or deletion may cause sex different effects on fertility. In summary, this study revealed the sexual similarities and dimorphic of higher-order chromatin architecture, homologous pairing and transcriptome during meiotic prophase of both oogenesis and spermatogenesis.
Overall design By crossing female C57BL/6NJ (mVenus-P2A-HA-Sycp3 transgene mice) to male PWK-PhJ mice, we successfully purified oocytes and spermatocytes at time-points enriched of specific meiotic prophase substages, with the help of FACS of SYCP3 signal from F1 hybrid mice (B6xPWK). Then we performed sisHi-C, RNA-Seq and ATAC-Seq.
Contributor(s) He J, Yan A
Citation(s) 37963468
Submission date Dec 07, 2022
Last update date Dec 23, 2023
Contact name Jing He
Phone +86-10-62795064
Organization name Tsinghua University
Department School of Medicine
Lab Stem cell and reproduction
Street address Room D110, Medical Science Building, School of Medicine, Tsinghua University
City Beijing
State/province Beijing
ZIP/Postal code 100084
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (44)
GSM6798033 B6xPWK_FPGC_hic_rep1
GSM6798034 B6xPWK_FPGC_hic_rep2
GSM6798035 B6xPWK_FLep_hic_rep1
BioProject PRJNA909605

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Supplementary file Size Download File type/resource
GSE220303_RAW.tar 6.6 Gb (http)(custom) TAR (of BW, HIC, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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