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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 01, 2023 |
| Title |
Single-cell analysis reveals dynamic changes of A549 cells in monolayer, A549 xenografts in nude mice, and xenografts after exposure to dacomitinib |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The in vitro A549 cells, and A549 xenografts in nude mouse were two classic and commonly used models for anti-cancer drug discovery. Here dynamic changes of the A549 models, and also A549 xenografts after exposure to dacomitinib were revealed by single-cell transcriptome analysis.
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| Overall design |
A549 cells in monolayer, A549 xenografts in nude mice, and xenografts after exposure to dacomitinib were digested and processed for single-cell RNA seqencing. Then bioinformatics analysis were performed basing on the transcriptome data.
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| Contributor(s) |
Chen X, Yu Y, Zheng H, Yang M, Cai Q, Zhang W, Jiang F, Zhu Y, Ma L, Xie W, Yang H, Zhang T, Huang G, Zhou Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 07, 2022 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Yu Yangziwei |
| E-mail(s) |
212302491@st.usst.edu.cn
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| Phone |
18116202232
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| Organization name |
Shanghai University of Medicine and Health Science
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| Street address |
上海市浦东新区周祝公路279号
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| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
201318 |
| Country |
China |
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| Platforms (1) |
| GPL25526 |
Illumina NovaSeq 6000 (Homo sapiens; Mus musculus) |
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| Samples (4)
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| GSM6801667 |
A549 cells, replicate 1, scRNAseq |
| GSM6801668 |
A549 xenografts, replicate 1, scRNAseq |
| GSM6801669 |
A549 xenografts exposed to 7.5mg/kg dacomitinib, replicate 1, scRNAseq |
| GSM6801670 |
A549 xenografts exposed to 15mg/kg dacomitinib, replicate 1, scRNAseq |
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| Relations |
| BioProject |
PRJNA909766 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220435_Combined_SC102_Expression_Data.st.txt.gz |
156.9 Mb |
(ftp)(http) |
TXT |
| GSE220435_Combined_SC102_RSEC_MolsPerCell.csv.gz |
45.5 Mb |
(ftp)(http) |
CSV |
| GSE220435_Combined_SC102_RSEC_ReadsPerCell.csv.gz |
56.6 Mb |
(ftp)(http) |
CSV |
| GSE220435_Combined_SC104_Expression_Data.st.txt.gz |
250.9 Mb |
(ftp)(http) |
TXT |
| GSE220435_Combined_SC104_RSEC_MolsPerCell.csv.gz |
74.5 Mb |
(ftp)(http) |
CSV |
| GSE220435_Combined_SC104_RSEC_ReadsPerCell.csv.gz |
83.8 Mb |
(ftp)(http) |
CSV |
| GSE220435_JZsc102_BKDL190811024-1a.txt.gz |
154 b |
(ftp)(http) |
TXT |
| GSE220435_SC102_Metrics_Summary.csv.gz |
880 b |
(ftp)(http) |
CSV |
| GSE220435_SC102_SC103_SampleTag_Status.csv.gz |
495 b |
(ftp)(http) |
CSV |
| GSE220435_SC102_Sample_Tag_Calls.csv.gz |
56.5 Kb |
(ftp)(http) |
CSV |
| GSE220435_SC102_UMI_Adjusted_Stats.csv.gz |
1.0 Mb |
(ftp)(http) |
CSV |
| GSE220435_SC104_Metrics_Summary.csv.gz |
877 b |
(ftp)(http) |
CSV |
| GSE220435_SC104_SC105_SampleTag_Status.csv.gz |
531 b |
(ftp)(http) |
CSV |
| GSE220435_SC104_Sample_Tag_Calls.csv.gz |
76.1 Kb |
(ftp)(http) |
CSV |
| GSE220435_SC104_UMI_Adjusted_Stats.csv.gz |
878.8 Kb |
(ftp)(http) |
CSV |
| GSE220435_hg19_Combined.rds.gz |
468.9 Mb |
(ftp)(http) |
RDS |
| GSE220435_hg19_Combined.subset.rds.gz |
164.4 Mb |
(ftp)(http) |
RDS |
| GSE220435_hg19_Combined_0.3.rds.gz |
370.3 Mb |
(ftp)(http) |
RDS |
| GSE220435_mm10_Combined.rds.gz |
20.2 Mb |
(ftp)(http) |
RDS |
| GSE220435_mm10_Combined_0.3.rds.gz |
20.3 Mb |
(ftp)(http) |
RDS |
| GSE220435_mm10_Combined_0.3.subset.rds.gz |
22.1 Mb |
(ftp)(http) |
RDS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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