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Series GSE22075 Query DataSets for GSE22075
Status Public on Jul 01, 2010
Title Genome-wide Chromatin Maps Derived from Limited Numbers of Hematopoietic Progenitors
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Whole genome mapping of protein-DNA interactions can be performed by coupling chromatin immunoprecipitation (ChIP) with high-throughput sequencing (ChIP-Seq). However, current methods require large amounts of starting materials which precludes their application to rare cell types. Here, we combine a high-sensitivity ChIP assay with a novel library preparation procedure to map histone modifications in as few as 10,000 cells. We apply the technique to acquire genome-wide chromatin maps for an enriched population of hematopoietic progenitors, and thereby gain insight into their developmental program.
Overall design An optimized ChIP protocol for small number of cells and a novel Librray preperation methods for high throughput sequencing of picograms amount of ChIP DNA.
Contributor(s) Adli M, Bernstein BE
Citation(s) 20622861
Submission date Jun 01, 2010
Last update date May 15, 2019
Contact name Jiang Zhu
Organization name Massachusetts General Hospital, Harvard Medical School and Broad Institute
Lab Bradley Bernstein Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (7)
GSM548821 H3K4me3 ChIP-Seq-10K ESC
GSM548822 H3K4me3 ChIP-Seq-LSK cells R1
GSM548823 H3K4me3 ChIP-Seq-LSK cells R2
SRA SRP002641
BioProject PRJNA128955

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22075_RAW.tar 3.8 Gb (http)(custom) TAR (of DAT, TXT, WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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