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Series GSE221106 Query DataSets for GSE221106
Status Public on Mar 14, 2023
Title Spatial analysis of microglial and vascular responses following recurrent intranasal Group A Streptococcus infections
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Other
Summary Peripheral infections can result in neurocognitive changes in many contexts, including after recurrent Group A Streptococcus (GAS) infections in children. To explore the underlying mechanisms, here we used an intranasal inoculation model to analyze nearly 200,000 cells from the mouse olfactory bulb (OB) by multiplexed error-robust fluorescent in situ hybridization (MERFISH). Upon repeated GAS inoculations, endothelial cells (ECs) responded by downregulating blood-brain barrier and extracellular matrix genes. Microglia upregulated response to interferon and antigen presentation genes and downregulated homeostatic microglia genes. We determined that microglia with higher expression of Streptococcus-responsive genes were localized to the outer glomerular layer of the OB, possibly due to their proximity to infiltrating CD4 T cells. Expression of homeostatic genes was correspondingly high in the inner granular layer of the OB.
Overall design Brains were isolated from P53 mice following five repeated inoculations with either a suspension of Group A Streptococcus or with vehicle (PBS), and profiled at 24 hours post-inoculation. After perfusion with cold PBS, brains were embedded in OCT for sectioning onto specialized coverslips. Coronal sections of the olfactory bulb (around bregma 4.28) were collected for analysis by MERFISH
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Contributor(s) Wayne CR, Faust TE, Durán-Laforet V, Schafer DP, Agalliu D
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Submission date Dec 15, 2022
Last update date Mar 14, 2023
Contact name Travis E. Faust
Organization name University of Massachusetts Chan Medical School
Department Neurobiology
Lab Schafer
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01655-0002
Country USA
Platforms (1)
Samples (8)
GSM6845429 OB_PBS_1
GSM6845430 OB_GAS_1
GSM6845431 OB_PBS_2
BioProject PRJNA912763

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Supplementary file Size Download File type/resource
GSE221106_220428_AgalliuLab_GAS_combat_AllCells_NoHybrids.rds.gz 458.4 Mb (ftp)(http) RDS
GSE221106_220428_AgalliuLab_GAS_combat_AllCells_norm.rds.gz 529.6 Mb (ftp)(http) RDS
GSE221106_220921_AgalliuLab_GAS_combat_MicrogliaOnly_TCellNNDs.rds.gz 20.3 Mb (ftp)(http) RDS
GSE221106_CountsAndCellMetadata_Coverslip_1.csv.gz 13.5 Mb (ftp)(http) CSV
GSE221106_CountsAndCellMetadata_Coverslip_2.csv.gz 11.7 Mb (ftp)(http) CSV
GSE221106_CountsAndCellMetadata_Coverslip_3.csv.gz 10.5 Mb (ftp)(http) CSV
GSE221106_CountsAndCellMetadata_Coverslip_4.csv.gz 16.6 Mb (ftp)(http) CSV
Processed data are available on Series record

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