GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE221140 Query DataSets for GSE221140
Status Public on Jun 05, 2024
Title Cistrome analyses of AR-V7.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Although AR-V7 has been intensively studied, it remains unclear whether AR-V7 can specifically activate a distinctive transcriptional program from the full-length AR (AR-FL), and whether AR-V7 may play a role in accelerating the metastatic progression of castration-resistant PCa (CRPC). In this study, we hypothesize that AR-V7 can drive a distinct transcription program from AR-FL in CRPC condition. To test this, we used LNCaP model with inducible overexpression of AR-V7 or AR-FL to examine the effects of AR-V7 overexpression or AR-FL overexpression stimulated with DHT for 4 hours on AR-V7 or AR-FL cistromes. We also studied the effects of AR-V7 on cistromes of pioneer factor FOXA1 and active histone marker H3K27ac, and the function of phosphorylation at Ser81 on AR-V7 function in CRPC.
Overall design ChIP-seq analyses of V5 tagged AR-V7, AR-C, FOXA1 and H3K27ac were performed to examine AR-V7, AR-C, FOXA1 and H3K27ac binding with or without overexpression of AR-V7 or AR-V7-S81A in LNCaP stable cell line under 5% charcoal-stripped FBS condition. The stable cells were generated by stably infecting LNCaP cells with tetracycline inducible overexpression of wild-type AR-V7 or S81A-AR-V7 virus. These stable cells were grown in the medium containing 10% tetracycline-free FBS.
Contributor(s) Han D, Labaf M, Zarringhalam K, Cai C
Citation(s) 38687617
Submission date Dec 16, 2022
Last update date Jun 05, 2024
Contact name Maryam Labaf
Organization name University of Massachusetts Boston
Department Biology
Lab Cai Lab
Street address 100 William T Morrissey Blvd
City Boston
State/province MA
ZIP/Postal code 02125
Country USA
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (24)
GSM6845897 ChIP-V5_LN-tet-ARV7_CSS_Veh_rep1
GSM6845898 ChIP-V5_LN-tet-ARV7_CSS_Veh_rep2
GSM6845899 ChIP-V5_LN-tet-ARV7_CSS_Dox_rep1
This SubSeries is part of SuperSeries:
GSE221142 Distinct activity of androgen receptor splice variants in promoting prostate cancer metastasis
BioProject PRJNA913106

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 175.1 Mb (ftp)(http) BW
GSE221140_ChIP-ARC_LN-tet-ARV7_CSS_DHT.narrowPeak.gz 441.8 Kb (ftp)(http) NARROWPEAK 192.5 Mb (ftp)(http) BW
GSE221140_ChIP-ARC_LN-tet-ARV7_CSS_Dox.narrowPeak.gz 28.0 Kb (ftp)(http) NARROWPEAK 191.0 Mb (ftp)(http) BW
GSE221140_ChIP-ARC_LN-tet-ARV7_CSS_Dox_DHT.narrowPeak.gz 224.4 Kb (ftp)(http) NARROWPEAK 231.0 Mb (ftp)(http) BW
GSE221140_ChIP-ARC_LN-tet-ARV7_CSS_Veh.narrowPeak.gz 33.2 Kb (ftp)(http) NARROWPEAK 227.6 Mb (ftp)(http) BW
GSE221140_ChIP-FOXA1_LN-tet-ARV7_CSS_Dox.narrowPeak.gz 2.1 Mb (ftp)(http) NARROWPEAK 259.2 Mb (ftp)(http) BW
GSE221140_ChIP-FOXA1_LN-tet-ARV7_CSS_Veh.narrowPeak.gz 2.1 Mb (ftp)(http) NARROWPEAK 209.2 Mb (ftp)(http) BW
GSE221140_ChIP-H3K27ac_LN-tet-ARV7_CSS_Dox.narrowPeak.gz 1.2 Mb (ftp)(http) NARROWPEAK 175.4 Mb (ftp)(http) BW
GSE221140_ChIP-H3K27ac_LN-tet-ARV7_CSS_Veh.narrowPeak.gz 1.1 Mb (ftp)(http) NARROWPEAK 26.3 Mb (ftp)(http) BW
GSE221140_ChIP-V5_LN-tet-ARV7-S81A_CSS_Dox.narrowPeak.gz 154.7 Kb (ftp)(http) NARROWPEAK 8.9 Mb (ftp)(http) BW
GSE221140_ChIP-V5_LN-tet-ARV7-S81A_CSS_Veh.narrowPeak.gz 7.4 Kb (ftp)(http) NARROWPEAK 25.0 Mb (ftp)(http) BW
GSE221140_ChIP-V5_LN-tet-ARV7_CSS_Dox.narrowPeak.gz 79.5 Kb (ftp)(http) NARROWPEAK 12.1 Mb (ftp)(http) BW
GSE221140_ChIP-V5_LN-tet-ARV7_CSS_Veh.narrowPeak.gz 2.2 Kb (ftp)(http) NARROWPEAK
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap