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| Status |
Public on Apr 30, 2023 |
| Title |
Evaluation of cell cycle phase dependent transcriptional changes by single cell RNA-seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed single cell RNA MULTI-seq using 10x v3 platform with 24h induced and non-induced FOXD3 KO cells.
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| Overall design |
FOXD3 loss was induced with 4HT for 24h (control was treated with EtOH) with two independently grown cultures per conditions. Cells were lipid barcoded according to McGinnis et al (2019) and stoichiometrically pooled. A total of 45920 cells were used as input to the 10x controller aiming for 20000 cells captured.
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| Contributor(s) |
Goekbuget D, Blelloch R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 19, 2022 |
| Last update date |
May 02, 2023 |
| Contact name |
Robert Blelloch |
| Organization name |
UCSF
|
| Department |
Urology
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| Street address |
35 Medical Center Way
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE183420 |
Transcriptional repressor FOXD3 safeguards genome integrity in embryonic stem cells |
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| Relations |
| BioProject |
PRJNA913699 |
