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Series GSE22140 Query DataSets for GSE22140
Status Public on Jun 05, 2010
Title Arthritic KRN T cells and wild type T cells from germ free and specific pathogen free mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary A general defect of GF K/BxN T cell proliferation response toward antigen motivated us to look for the impairment in GF K/BxN T cells that might leads to the low Ab production and reduced disease phenotype seen in GF K/BxN mice. To find the difference between GF and SPF K/BxN T cells in a broad and non-biased fashion, we performed gene-expression profiling of these cells using microarrays.
 
Overall design All gene expression profiles were obtained from highly purified T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled for sorting, and triplicates or quadruplates were generated for all groups. Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
 
Contributor(s) Wu H, Mathis D, Benoist C
Citation(s) 20620945
Submission date Jun 04, 2010
Last update date Feb 11, 2019
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (13)
GSM550706 GF BXN#1
GSM550707 GF BXN#2
GSM550708 GF BXN#3
Relations
BioProject PRJNA127581

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22140_RAW.tar 53.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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