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| Status |
Public on Jan 16, 2023 |
| Title |
Transcriptional Response of Multi-stress Tolerant Saccharomyces cerevisiae to Sequential Stress |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To better understand how yeast adapt and respond to sequential stressors, an industrial yeast strain, URM 6670 (also known as BT0510), which is highly flocculent, tolerant to ethanol, osmotic and heat shock stresses, was subjected to three different treatments: 1. osmotic stress followed by ethanol stress, 2. oxidative stress followed by ethanol stress, 3. glucose withdrawal followed by ethanol stress. Samples were collected before the first stress (control), after the first stress and after the second stress (ethanol). RNA was extracted and analyzed by RNAseq.
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| Overall design |
Comparison of the transcriptional responses, relative to untreated samples, of yeast strain BT0510 subjected to oxidative stress, osmotic stress and glucose deprivation stress, followed in all cases by ethonal exposure
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| Contributor(s) |
Broach JR, Costa AC, Russo M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 22, 2022 |
| Last update date |
Jan 18, 2023 |
| Contact name |
James R Broach |
| E-mail(s) |
jbroach@hmc.psu.edu
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| Organization name |
Pennsylvania State University College of Medicine
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| Department |
Biochemistry and Molecular Biology
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| Street address |
700 HMC Crescent Rd
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| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
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| Platforms (1) |
| GPL27812 |
Illumina NovaSeq 6000 (Saccharomyces cerevisiae) |
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| Samples (27)
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| GSM6886468 |
BT0510, Osmotic stress - control, biological replicate 1 |
| GSM6886469 |
BT0510, Osmotic stress - osmotic stress, biological replicate 1 |
| GSM6886470 |
BT0510, Osmotic stress - alcohol stress, biological replicate 1 |
| GSM6886471 |
BT0510, Osmotic stress - control, biological replicate 2 |
| GSM6886472 |
BT0510, Osmotic stress - osmotic stress, biological replicate 2 |
| GSM6886473 |
BT0510, Osmotic stress - alcohol stress, biological replicate 2 |
| GSM6886474 |
BT0510, Osmotic stress - control, biological replicate 3 |
| GSM6886475 |
BT0510, Osmotic stress - osmotic stress, biological replicate 3 |
| GSM6886476 |
BT0510, Osmotic stress - alcohol stress, biological replicate 3 |
| GSM6886477 |
BT0510, Oxidative stress - control, biological replicate 1 |
| GSM6886478 |
BT0510, Oxidative stress - oxidative stress, biological replicate 1 |
| GSM6886479 |
BT0510, Oxidative stress 1 - alcohol stress, biological replicate 1 |
| GSM6886480 |
BT0510, Oxidative stress - control, biological replicate 2 |
| GSM6886481 |
BT0510Oxidative stress 2 - oxidative stress, biological replicate 2 |
| GSM6886482 |
BT0510, Oxidative stress - alcohol stress, biological replicate 2 |
| GSM6886483 |
BT0510, Oxidative stress - contro, biological replicate 3l |
| GSM6886484 |
BT0510, Oxidative stress - oxidative stress, biological replicate 3 |
| GSM6886485 |
BT0510, Oxidative stress - alcohol stress, biological replicate 3 |
| GSM6886486 |
BT0510, glucose stress - control, biological replicate 1 |
| GSM6886487 |
BT0510, glucose stress - glucose stress, biological replicate 1 |
| GSM6886488 |
BT0510, glucose stress - alcohol stress, biological replicate 1 |
| GSM6886489 |
BT0510, glucose stress - control, biological replicate 2 |
| GSM6886490 |
BT0510, glucose stress - glucose stress, biological replicate 2 |
| GSM6886491 |
BT0510, glucose stress - alcohol stress, biological replicate 2 |
| GSM6886492 |
BT0510, glucose stress - control, biological replicate 3 |
| GSM6886493 |
BT0510, glucose stress - glucose stress, biological replicate 3 |
| GSM6886494 |
BT0510, glucose stress - alcohol stress, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA915019 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221570_RAW.tar |
4.2 Mb |
(http)(custom) |
TAR (of DIFF) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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