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| Status |
Public on Dec 01, 2023 |
| Title |
Gene expression profile at single cell level of CD45+ cells from AB1 mesothelioma treated with either vaccinia virus Tiantan strain or mock-treated |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the immune infiltrates in the tumor microenvironment in response to vaccinia virotherapy, we used single cell RNA sequencing (scRNAseq) to analyze the diversity of total CD45+ isolated from AB1 mesothelioma
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| Overall design |
Balb/c mice bearing 7-day-old subcutaneous AB1 tumors were intratumorally treated with either modified vaccinia virus Tiantan strain (MVTT) or mock-treated, total tumor-infiltrating CD45+ cells were sorted by flow cytometry 2 days post treatment and processed in the BD Rhapsody system for scRNA-seq
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| Contributor(s) |
Tan Z, Chen Z |
| Citation(s) |
38066571 |
| |
| Submission date |
Dec 23, 2022 |
| Last update date |
Dec 11, 2023 |
| Contact name |
Zhiwu Tan |
| E-mail(s) |
zwtan@hku.hk
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| Organization name |
University of Hong Kong
|
| Department |
Microbiology
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| Lab |
AIDS Institute
|
| Street address |
21 Sassoon Road, Pokfulam
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| City |
Hong Kong |
| ZIP/Postal code |
000000 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA915321 |
