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Series GSE221718 Query DataSets for GSE221718
Status Public on Dec 03, 2023
Title Identification of potential miRNA–mRNA regulatory network contributing to cytarabine resistance HL60 cells [array]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cytarabine is the main drug for acute myeloid leukemia (AML) treatment; however, drug resistance hinders the treatment of AML. Although microRNA (miRNA) alteration is one of the well-recognized mechanisms underlying drug resistance in AML, few studies have investigated the role and function of miRNAs in the development of cytarabine resistance. In this study, total RNA was isolated from parental HL60 and cytarabine resistant HL60 (R-HL60) cells. Subsequently, miRNAs and mRNAs were detected using small RNA sequencing and gene expression array, respectively. The miRNAs and genes with ≥ 2-fold difference in expression between HL60 and R-HL60 cells were screened out. Negatively correlated miRNA–mRNA pairs were selected as candidate miRNA–mRNA target pairs by using the miRDB, Targetscan or miRTar databases. Functional enrichment analysis of differentially expressed genes (DEGs) included in the candidate miRNA–mRNA network was performed. The results revealed that CCL2, SOX9, SLC8A1, ICAM1, CXCL10, SIPR2, FGFR1, OVOL2, MITF, and CARD10 were simultaneously involved in seven GO pathways, namely the regulation of cell migration, regulation of locomotion, regulation of cellular component movement, cell migration, locomotion, cell motility, localization of cell. These genes were negatively correlated with the altered miRNAs (miR-1-3p, miR-155-5p, miR-1255b-5p, miR-200c-5p, miR-3609, miR-1285-3p, miR-124-3p, miR-146a-5p, miR-497a-5p, and miR-3150a-5p), suggesting that they are the potential targets of the miRNAs to regulate cell migration behavior or ability. Therefore, our results advance our understanding of the regulatory mechanism underlying cytarabine resistance development, specifically related to miRNAs.
 
Overall design Total RNA was isolated from parental HL60 and cytarabine resistant HL60 (R-HL60) cells. Subsequently, mRNAs were detected using gene expression array.
 
Contributor(s) Tseng YH
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Submission date Dec 24, 2022
Last update date Dec 04, 2023
Contact name Yu-Hsin Tseng
E-mail(s) grapepuff@gmail.com
Organization name Kaohsiung Medical University Hospital
Department Department of Pediatrics
Street address No.100, Tzyou 1st Rd., Sanmin Dist.
City Kaohsiung
ZIP/Postal code 80756
Country Taiwan
 
Platforms (1)
GPL25261 Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Gene Name Version]
Samples (2)
GSM6893698 parental HL60 cells [array]
GSM6893699 cytarabine resistance HL60 cells [array]
This SubSeries is part of SuperSeries:
GSE221719 Identification of potential miRNA–mRNA regulatory network contributing to cytarabine resistance HL60 cells
Relations
BioProject PRJNA915607

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE221718_RAW.tar 1.0 Mb (http)(custom) TAR (of TXT)
GSE221718_normalized_data.txt.gz 806.1 Kb (ftp)(http) TXT
Processed data are available on Series record

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