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| Status |
Public on Dec 01, 2023 |
| Title |
Mitochondrial-Targeted Antioxidant Attenuates Preeclampsia-Like Phenotypes Induced by Syncytiotrophoblast-Specific Gαq Signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This experiment explored the placental transcriptomic effects of syncytiotrophoblast-specific Gαq signaling at gestational day 14.5 in mice and assessed whether these responses were altered upon administration of a mitochondrial-targeted antioxidant.
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| Overall design |
Activation of the Gαq cascade in syncytiotrophoblast cells was achieved by crossing dams harboring a Cre-dependent Gαq-coupled DREADD (hM3Dq) with Gcm1-Cre+/- sires to elicit syncytiotrophoblast-restricted hM3Dq expression in ~50% of placentas within a dam. The synthetic DREADD ligand clozapine N-oxide (CNO; 2 mg/kg/d, ip) or saline (2 mL/kg/d, ip) was administered mid-gestation (gestational days 12.5-14.5) before the profiling of placenta gene expression at gestational day 14.5. In a separate cohort of double transgenic (hM3Dq+, Gcm1-Cre+) pregnancies, the mitochondrial-targeted antioxidant, Mitoquinone (MitoQ; 5 mg/kg/d, ip), was co-administered with CNO.
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| Contributor(s) |
Opichka MA, Kwitek AE, Sigmund CD, McIntosh JJ, Grobe JL |
| Citation(s) |
38039359 |
| |
| Submission date |
Dec 24, 2022 |
| Last update date |
Dec 13, 2023 |
| Contact name |
Justin L Grobe |
| E-mail(s) |
jgrobe@mcw.edu
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| Phone |
(414) 955-4981
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| Organization name |
Medical College of Wisconsin
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| Department |
Physiology
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| Street address |
8701 W Watertown Plank Road
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| City |
Milwaukee |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA915630 |
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