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Status |
Public on Mar 27, 2024 |
Title |
Gene expression profile at single cell level of Paneth cells from the small intestine upon genetic targeting and in inflammatory conditions |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We combined lineage tracing with single cell RNA sequencing (scRNA-seq) to study the mechanisms underlying the conversion of Paneth cells into bona fide tumor cells.
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Overall design |
Crypts were purified from the small intestine, and enzymatically dissociated with TryplE to obtain a single cell suspension. Cells were stained with fluorescent antibodies (Cd24, cKit) and with Hashing antibodies. Paneth cells were enriched by Fluorescence-activated cell sorting (FACS) according to the SSChiCD24hicKithi gate or by the presence of Yfp/td-Tomato signal. Sorted cells from several mice (controls + genetic targeting) were mixed and processed as Paneth Pool (PP) with 10X Gene expression profiling technology with feature barcoding).
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Web link |
https://www.nature.com/articles/s41588-024-01801-y
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Contributor(s) |
Verhagen MP, Joosten R, Sacchetti A, Fodde R |
Citation(s) |
38902475 |
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Submission date |
Dec 27, 2022 |
Last update date |
Jun 24, 2024 |
Contact name |
Mathijs Verhagen |
E-mail(s) |
m.p.verhagen@erasmusmc.nl
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Organization name |
Erasmus Medical Center
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Street address |
Doctor Molewaterplein 40
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City |
Rotterdam |
ZIP/Postal code |
3015 GD |
Country |
Netherlands |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (5)
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Relations |
BioProject |
PRJNA916251 |