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Series GSE222657 Query DataSets for GSE222657
Status Public on Jan 01, 2024
Title CONSTANS alters the circadian clock in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment. Among these processes, the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks. The photoperiodic pathway, which controls the plant response to day length, is one of the most important pathways controlling flowering. In Arabidopsis photoperiodic flowering, CONSTANS (CO) is the central gene activating the expression of the florigen FLOWERING LOCUS T (FT) in the leaves at the end of a long day. CO expression is strongly regulated by the circadian clock. However, to date, no evidence has been reported regarding a feedback loop from the photoperiod pathway back to the circadian clock. Using transcriptional networks, we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod pathway. Gene expression, chromatin immunoprecipitation experiments and phenotypic analysis allowed us to elucidate the role of CO over the circadian clock. Plants with altered CO expression showed a different internal clock period, measured by daily rhythmic movements in the leaves. We show that CO is able to activate key genes related to the circadian clock, such as CCA1, LHY, PRR5 and GI, at the end of a long day by binding to specific sites on their promoters. Moreover, a significant number of PRR5 repressed target genes are upregulated by CO, and this could explain the phase transition promoted by CO. The CO-PRR5 complex interacts with the bZIP transcription factor HY5 and helps to localize the complex in the promoters of clock genes. Our results indicate that there may be a feedback loop in which CO communicates back to the circadian clock, feeding seasonal information to the circadian system.
 
Overall design Chromatin immunoprecipitation (ChIP-seq) for CONSTANS in Arabidopsis. Seedlings were grown in LD and harvested at 7DAG. CO ovexpressing lines were used to get high protein amount. CO mutants (co-10) were employed as control. The immunoprecipitation was carried out using specific anti-CO antibodies
 
Contributor(s) de los Reyes P, Romero-Campero FJ, Gao. H, Serrano-Bueno G, Romero JM, Coupland G, Valverde F
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Submission date Jan 11, 2023
Last update date Jan 01, 2024
Contact name Pedro de los Reyes
E-mail(s) pedro.reyes@ibvf.csic.es
Organization name CSIC
Department Institute for Plant Biochemistry and Photosynthesis
Street address c/Americo Vespucio 49
City Sevilla
ZIP/Postal code 41092
Country Spain
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (8)
GSM6928388 Input of 35SCO, rep1
GSM6928389 Input of 35SCO, rep2
GSM6928390 Input of co-10, rep1
Relations
BioProject PRJNA922974

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE222657_35SCO_peaks.narrowPeak.gz 84.2 Kb (ftp)(http) NARROWPEAK
GSE222657_co10_peaks.narrowPeak.gz 9.8 Kb (ftp)(http) NARROWPEAK
GSE222657_filtered_peaks.narrowPeak.gz 81.0 Kb (ftp)(http) NARROWPEAK
GSE222657_input_35SCO.bw 38.5 Mb (ftp)(http) BW
GSE222657_input_co10.bw 33.4 Mb (ftp)(http) BW
GSE222657_ip_35SCO.bw 16.2 Mb (ftp)(http) BW
GSE222657_ip_co10.bw 8.7 Mb (ftp)(http) BW
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Raw data are available in SRA
Processed data are available on Series record

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