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Series GSE22324 Query DataSets for GSE22324
Status Public on Jun 14, 2010
Title Mapping of disease-associated expression polymorphisms in primary peripheral blood CD4+ lymphocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Analysis of expression quantitative trait loci (eQTLs) using RNA derived from freshly harvested peripheral blood CD4+ lymphocytes from 200 asthmatics collected in clinical settings.
Overall design RNA was obtained from peripheral blood CD4+ lymphocytes at four study centers. Peripheral blood (17 cc) was collected into BD Vacutainer CPT tubes (BD Diagnostics, Franklin Lakes, New Jersey) and placed on ice. Samples were centrifuged within 1 hour of collection for 20 minutes at 1700RCF, followed by mononuclear cell layer isolation and suspension in 10 ml of PBS. We isolated CD4+ lymphocytes using anti-CD4+ microbeads by column separation (Miltenyi Biotec, Auburn, CA) using 20 ┬Ál anti-CD4+ Micro beads per 106 total cells
Contributor(s) Raby BA
Citation(s) 20833654, 27494826, 21254216, 31992292, 37275375
Submission date Jun 14, 2010
Last update date Aug 25, 2023
Contact name Benjamin A Raby
Organization name Channing Laboratory
Street address 181 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL6104 Illumina humanRef-8 v2.0 expression beadchip
Samples (200)
GSM555647 Subject 1
GSM555648 Subject 2
GSM555649 Subject 3
BioProject PRJNA127537

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22324_RAW.tar 3.4 Mb (http)(custom) TAR
GSE22324_non-normalized_data.txt.gz 17.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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