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Series GSE223408 Query DataSets for GSE223408
Status Public on Oct 02, 2023
Title Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Mouse Array]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Pancreatic ductal adenocarcinoma (PDAC) tumours carry multiple gene mutations and respond poorly to treatments. There is currently an unmet need for drug carriers that can deliver multiple gene cargoes to high solid tumour burden like PDAC. Here, we report a dual-targeted extracellular vesicle (EV) carrying high loads of RNA that effectively suppresses large PDAC tumours in mice. The EV surface contains a CD64 protein that has a tissue targeting peptide and a humanized monoclonal antibody. Cells sequentially transfected with plasmid DNAs encoding for the RNA and protein of interest by Transwell®-based asymmetric cell electroporation released abundant targeted EVs with high RNA loading. Together with a low dose chemotherapy drug, Gemcitabine, dual-targeted EVs effectively suppressed large orthotopic PANC-1 and patient derived xenograft tumours in mice and extended animal survival. This work presents a clinically accessible and scalable way to produce abundant EVs for delivering multiple gene cargoes to large solid tumours.
Overall design MOUSE_arrays
Contributor(s) Wang K, Lee I, Fallen S, Lee L, Chiang N
Citation(s) 37872156
Submission date Jan 20, 2023
Last update date Nov 13, 2023
Contact name Inyoul Lee
Organization name Institute for Systems Biology
Street address 401 Terry Ave. North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platforms (1)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (14)
GSM6947333 Gene expression of Plasmids cd64_TP53 treated MEF-derived EV_rep3
GSM6947334 Gene expression of Plasmids cd64_TP53 treated MEF-derived EV_rep1
GSM6947335 Gene expression of PBS treated MEF-derived EV_rep2
This SubSeries is part of SuperSeries:
GSE223409 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer
BioProject PRJNA925942

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223408_RAW.tar 175.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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