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Series GSE22352 Query DataSets for GSE22352
Status Public on Mar 31, 2012
Title Identification of LEC1 target genes
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by array
Summary The transcription factor (TF) LEAFY COTYLEDON1 (LEC1) acts as an essential regulator of Arabidopsis embryogenesis and seed development. It controls aspects of early embryogenesis such as cotyledon identity and suspensor morphology, as well as seed maturation processes such as storage compound accumulation, acquisition of desiccation tolerance and dormancy.
To identify downstream components of the LEC1 regulon, dexamethasone-regulated expression of LEC1 and ChIP/chip were applied and revealed the enrichment of phytohormone- as well as elongation-related genes among LEC1 target genes.
Overall design Two weeks old 35S::LEC1::GR seedlings were treated with DEX or ethanol for 24 hours before cross-linking and chromatin isolation. Four biological replicates were performed, using an ethanol-treated sample as control (LEC1_EtOH versus LEC1_DEX). After data normalization, statistical analysis and exclusion of putative false negatives, a number of 356 target promoter regions were identified.
Contributor(s) Junker A, Moenke G, Haehnel U, Altschmied L, Seifert M
Citation(s) 22429691
Submission date Jun 15, 2010
Last update date Jan 08, 2013
Contact name Astrid Junker
Organization name Leibniz Institute for Plant Genetics and Crop Plant Research
Department Molecular Genetics
Street address Corrensstrasse 3
City Gatersleben
ZIP/Postal code 06466
Country Germany
Platforms (2)
GPL10543 Systemic Analysis of Araboidopsis Promoters (SAP, Benhamed et al. 2008) [Membrane A]
GPL10544 Systemic Analysis of Araboidopsis Promoters (SAP, Benhamed et al. 2008) [Membrane B]
Samples (16)
GSM556344 LEC1::GR uninduced 1, membrane A
GSM556345 LEC1::GR induced 1, membrane A
GSM556346 LEC1::GR uninduced 2, membrane A
BioProject PRJNA127493

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MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22352_GSM556344_GSM556351_ratio_data.txt.gz 197.7 Kb (ftp)(http) TXT
GSE22352_GSM556352_GSM556359_ratio_data.txt.gz 184.5 Kb (ftp)(http) TXT
GSE22352_RAW.tar 3.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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