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| Status |
Public on Mar 31, 2012 |
| Title |
Identification of LEC1 target genes |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by array
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| Summary |
The transcription factor (TF) LEAFY COTYLEDON1 (LEC1) acts as an essential regulator of Arabidopsis embryogenesis and seed development. It controls aspects of early embryogenesis such as cotyledon identity and suspensor morphology, as well as seed maturation processes such as storage compound accumulation, acquisition of desiccation tolerance and dormancy.
To identify downstream components of the LEC1 regulon, dexamethasone-regulated expression of LEC1 and ChIP/chip were applied and revealed the enrichment of phytohormone- as well as elongation-related genes among LEC1 target genes.
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| Overall design |
Two weeks old 35S::LEC1::GR seedlings were treated with DEX or ethanol for 24 hours before cross-linking and chromatin isolation. Four biological replicates were performed, using an ethanol-treated sample as control (LEC1_EtOH versus LEC1_DEX). After data normalization, statistical analysis and exclusion of putative false negatives, a number of 356 target promoter regions were identified.
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| Contributor(s) |
Junker A, Moenke G, Haehnel U, Altschmied L, Seifert M |
| Citation(s) |
22429691 |
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| Submission date |
Jun 15, 2010 |
| Last update date |
Jan 08, 2013 |
| Contact name |
Astrid Junker |
| Organization name |
Leibniz Institute for Plant Genetics and Crop Plant Research
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| Department |
Molecular Genetics
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| Street address |
Corrensstrasse 3
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| City |
Gatersleben |
| ZIP/Postal code |
06466 |
| Country |
Germany |
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| Platforms (2) |
| GPL10543 |
Systemic Analysis of Araboidopsis Promoters (SAP, Benhamed et al. 2008) [Membrane A] |
| GPL10544 |
Systemic Analysis of Araboidopsis Promoters (SAP, Benhamed et al. 2008) [Membrane B] |
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| Samples (16)
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| GSM556347 |
LEC1::GR induced 2, membrane A |
| GSM556348 |
LEC1::GR uninduced 3, membrane A |
| GSM556349 |
LEC1::GR induced 3, membrane A |
| GSM556350 |
LEC1::GR uninduced 4, membrane A |
| GSM556351 |
LEC1::GR induced 4, membrane A |
| GSM556352 |
LEC1::GR uninduced 1, membrane B |
| GSM556353 |
LEC1::GR induced 1, membrane B |
| GSM556354 |
LEC1::GR uninduced 2, membrane B |
| GSM556355 |
LEC1::GR induced 2, membrane B |
| GSM556356 |
LEC1::GR uninduced 3, membrane B |
| GSM556357 |
LEC1::GR induced 3, membrane B |
| GSM556358 |
LEC1::GR uninduced 4, membrane B |
| GSM556359 |
LEC1::GR induced 4, membrane B |
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| Relations |
| BioProject |
PRJNA127493 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22352_GSM556344_GSM556351_ratio_data.txt.gz |
197.7 Kb |
(ftp)(http) |
TXT |
| GSE22352_GSM556352_GSM556359_ratio_data.txt.gz |
184.5 Kb |
(ftp)(http) |
TXT |
| GSE22352_RAW.tar |
3.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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