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Series GSE223646 Query DataSets for GSE223646
Status Public on May 25, 2023
Title Compartmentalization of human B cell responses in early life through induction of bronchus-associated lymphoid tissue
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Infants and young children are more susceptible to common respiratory pathogens compared to adults, but can fare better against novel pathogens like SARS-CoV-2. The mechanisms by which infants and young children mount effective responses to respiratory pathogens are unknown. Here, we demonstrate through study of lungs and lung-associated lymph nodes (LLN) from infant and pediatric organ donors, aged 0-13 years, that bronchus-associated lymphoid tissue (BALT), develops in lungs during the first year of life. BALT structures, consisting of B cell follicles and T cell zones, increase in numbers in the early years, and subsequently decrease over childhood coincident with accumulation of memory T cells in the lung. Early life BALT contains germinal centers and supports B cell differentiation, clonal expansion, somatic hypermutation, and immunoglobulin class switching. High dimensional flow cytometry reveals seeding of lungs by newly formed B cells (transitional cells) during infancy coincident with the timing of maximal BALT formation. We further demonstrate increased lung-localized B cell responses during respiratory virus infection in infants. Together, our findings provide novel evidence for BALT as an early life adaptation for mobilizing in situ immune protection to the diverse respiratory challenges during this formative life stage.
Overall design CITE-seq/scBCR-seq
Contributor(s) Matsumoto R, Gray J, Sims PA, Farber DL
Citation(s) 37460638
Submission date Jan 24, 2023
Last update date Aug 24, 2023
Contact name Peter A Sims
Organization name Columbia University
Street address 3960 Broadway, Lasker 203AC
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (2)
GPL21697 NextSeq 550 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (24)
GSM6969908 Pooled ADT Library 1 for Donors HDL153, HDL141, HDL142
GSM6969909 Pooled ADT Library 2 for Donors HDL153, HDL141, HDL142
GSM6969910 Pooled ADT Library 3 for Donors HDL153, HDL141, HDL142
BioProject PRJNA927026

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223646_JoshGC.adt.matrix.txt.gz 6.2 Mb (ftp)(http) TXT
GSE223646_JoshGC.bcr.filtered_contig_annotations.csv.gz 8.3 Mb (ftp)(http) CSV
GSE223646_JoshGC.gex.matrix.txt.gz 102.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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