|
| Status |
Public on Dec 15, 2013 |
| Title |
Genome-wide Analysis of Enterococcus faecalis OG1RF Gene Expression During Infection |
| Platform organism |
Enterococcus faecalis V583 |
| Sample organism |
Enterococcus faecalis OG1RF |
| Experiment type |
Expression profiling by array
|
| Summary |
Changes in Enterococcus faecalis OG1RF gene expression during infection in a rabbit model of subdermal abscess formation were studied using microarray analysis.
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| |
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| Overall design |
Samples assayed in arrays consisted of the input inoculum and total RNA collected from the subdermal chambers at 2 and 8 hours post-inoculation. Alexa647-labeled samples were co-hybridized with 0.5 micrograms of sheared OG1RF genomic DNA labeled with Cy3 as a standard reference between chips. Four biological replicates were performed for each of the 3 time points.
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| |
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| Contributor(s) |
Frank KL, Grindle SM, Dunny GM |
| Citation(s) |
25545155 |
| |
| Submission date |
Jun 16, 2010 |
| Last update date |
Apr 23, 2015 |
| Contact name |
Kristi L Frank |
| Organization name |
University of Minnesota
|
| Department |
Dept. of Microbiology
|
| Lab |
Gary Dunny, PhD
|
| Street address |
420 Delaware St SE, MMC196
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55421 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10534 |
UnivMinnesota/Dunny Efaecalis v1.0 |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA127627 |
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