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Series GSE223913 Query DataSets for GSE223913
Status Public on Feb 05, 2024
Title Pol II pausing is a milestone on the road to complex animals
Organisms Escherichia coli; Haloferax mediterranei; Daphnia pulex; Strongylocentrotus purpuratus; Mus musculus; Perkinsus marinus; Dryas iulia; Dictyostelium discoideum; Nematostella vectensis; Sphaeroforma arctica; Capsaspora owczarzaki; Creolimax fragrantissima
Experiment type Other
Summary PRO-seq experiments in multiple organisms. Also, PRO-seq experiment of Nelfb and Nelfe degron mESC in serum/LIF with or without dTAG-13. 
Overall design We used Precision Run-On and Sequencing (PRO-seq) to study transcription in 20 extant organisms that represent very diverse branches on the tree of life, including multiple species near the base of the Metazoa phylogeny and the transition between plants and animals. We also used PRO-seq to study mESCs after degrading NELF-B or NELF-E.
Contributor(s) Chivu AG, Lewis JJ, Lis JT, Danko CG
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Submission date Jan 28, 2023
Last update date Feb 05, 2024
Contact name Alexandra Georgiana Chivu
Phone 6072624972
Organization name Cornell University
Department Molecular Biology and Genetics
Lab Danko and Lis labs
Street address 215 Tower Rd
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (12)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL21222 Illumina NextSeq 500 (Escherichia coli)
GPL23029 Illumina NextSeq 500 (Nematostella vectensis)
Samples (46)
GSM7000647 Escherichia_coli_c1
GSM7000648 Escherichia_coli_c2
GSM7000650 Escherichia_coli_c3
BioProject PRJNA929029

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223913_Extant_bigwig.tar.gz 229.1 Mb (ftp)(http) TAR
GSE223913_mESCs_NELF-B_bigwig.tar.gz 510.8 Mb (ftp)(http) TAR
GSE223913_mESCs_NELF-E_bigwig.tar.gz 530.0 Mb (ftp)(http) TAR
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Raw data are available in SRA
Processed data are available on Series record

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