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Series GSE224549 Query DataSets for GSE224549
Status Public on Apr 30, 2023
Title Loss of interleukin-1 beta is not protective in the lupus-prone NZM2328 mouse model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA-sequencing analysis identified upregulation of TNF and IL-17 signaling pathways specifically in females lacking IL-1β. Increases in these signaling pathways are also found in female patients with lupus nephritis, suggesting clinical relevance for upregulation of these pathways.
 
Overall design To better understand the impact of IL-1β deletion on lupus kidneys, genome-wide transcriptome analysis using RNA-sequencing (RNA-seq) was performed and transcriptional differences in the kidneys of male and female NZM and NZM-Il1b-/- mice were examined. Kidney RNA was isolated via Zymo Direct-zol RNA isolation kit and libraries were generated using standard poly-A prep kits (New England Biolabs) with the assistance of the U-M Advanced Genomics Core.
 
Contributor(s) Kahlenberg JM, Estadt SN, Tsoi LC
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Submission date Feb 05, 2023
Last update date Apr 30, 2023
Contact name Lam Tsoi
E-mail(s) alextsoi@umich.edu
Organization name University of Michigan
Street address Medical Sciences 1 Bldg. 1301 E. Catherine
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (14)
GSM7025819 1792-JL-1
GSM7025820 1792-JL-15
GSM7025821 1792-JL-16
Relations
BioProject PRJNA931738

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE224549_readcount.txt.gz 666.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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