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| Status |
Public on Jun 08, 2023 |
| Title |
Lactate-dependent Transcriptional Regulation Controls Mammalian Eye Morphogenesis [H3K27ac ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Mammalian retinal metabolism favors aerobic glycolysis. However, the role of glycolytic metabolism in retinal morphogenesis remains unknown. We report that aerobic glycolysis is necessary for the early stages of retinal development. Taking advantage of an unbiased approach that combines the use of eye organoids and single-cell RNA sequencing, we identify specific glucose transporters and glycolytic genes in retinal progenitors. Next, we determine that the optic vesicle territory of mouse embryos displays elevated levels of glycolytic activity. At the functional level, we show that removal of Glucose transporter 1 and Lactate dehydrogenase A gene activity from developing retinal progenitors arrests eye morphogenesis. Surprisingly, we uncover that lactate-mediated upregulation of key eye-field transcription factors is controlled by the epigenetic modification of histone H3 acetylation through histone deacetylase activity. Our results identify an unexpected bioenergetic independent role of lactate as a signaling molecule necessary for mammalian eye morphogenesis.
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| Overall design |
Comparative histone h3 k27 acetylation (H3K27ac) profiling analysis of ChIP-seq data for the eye organoids and its treatments (control, GNE-140, and GNE-140 with lactate).
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| Contributor(s) |
Takata N, Morgan MA, Rosenstadt ZM, Embry R, Schipma M |
| Citation(s) |
37452018 |
| |
| Submission date |
Feb 06, 2023 |
| Last update date |
Sep 07, 2023 |
| Contact name |
NOZOMU TAKATA |
| E-mail(s) |
nozomu.takata@northwestern.edu
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| Organization name |
Northwestern University Feinberg School of Medicine
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| Department |
Center for Vascular and Developmental Biology
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| Street address |
303 East Superior Street, 10-220
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| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE202759 |
Lactate-dependent Transcriptional Regulation Controls Mammalian Eye Morphogenesis. |
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| Relations |
| BioProject |
PRJNA932003 |
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