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Series GSE22465 Query DataSets for GSE22465
Status Public on Jan 03, 2012
Title Global transcriptomic profiling of lactacystin-mediated neuronal death
Organism Mus musculus
Experiment type Expression profiling by array
Summary Inhibition of proteasome degradation pathway has been implicated in neuronal cell death leading to neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease. Pharmacological proteasomal inhibitors such as lactacystin can induce apoptosis in cultured mouse cortical neurons through the activation of caspase-3. Furthermore, proteasomal inhibitors are also reported to mediate deleterious alterations in cell cycle regulation, inflammatory processes and protein aggregation and trigger the cell death pathway.
We discovered by microarray analysis that lactacystin treatment modulates the expression of both potentially neuroprotective as well as pro-apoptotic genes in neurons. However, the genes, upon transcriptional modulation, contribute to proteasomal inhibition-induced apoptosis, remains unidentified. By employing microarray analysis to decipher the time-dependent changes in transcription of these genes in cultured cortical neurons, we discovered different groups of genes were transcriptionally regulated at different phases of lactacystin-induced cell death.
Overall design Microarray analysis was carried out using 10 murine genome U74A and U74Av2 Genechips array (Affymetrix, Santa Clara, CA). The assignment of the arrays (GeneChip) was as follows: Control at 24 h (n=2), 48 h (n=2); exposure to 1 μM lactacystin for 24 h (n=3) and 48 h (n=3).
Contributor(s) Cheung NS, Chen MJ, Peng ZF, Manikandan J
Citation(s) 20683911
Submission date Jun 21, 2010
Last update date Feb 18, 2018
Contact name Minghui Jessica Chen
Organization name Menzies Research Institute
Department Neuroscience group
Lab A/P Steve Cheung
Street address Menzies Research Institute, University of Tasmania, Private Bag 24
City Hobart
State/province Tasmania
ZIP/Postal code 7001
Country Australia
Platforms (2)
GPL32 [MG_U74A] Affymetrix Murine Genome U74A Array
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
Samples (10)
GSM558054 Cortical neurons-Ctrl-24h-Rep 2
GSM558055 Cortical neurons-Ctrl-48h-Rep 2
GSM558056 Cortical neurons-Lact-24h-Rep 2
BioProject PRJNA128621

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Supplementary file Size Download File type/resource
GSE22465_RAW.tar 28.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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