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| Status |
Public on Feb 07, 2023 |
| Title |
Physiological and Molecular Characterization of an Boron-Resistant Saccharomyces cerevisiae Strain Obtained by Evolutionary Engineering |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
One of the essential or beneficial micronutrient for plants and animals is boron that is an ultra-trace element. Although boron can inhibit the growth of Saccharomyces cerevisiae around 80 mM, it is also a growth supplement. However, little information is currently available regarding the molecular mechanisms and essentiality of boron. In this paper, the approach was to generate S. cerevisiae mutants with high boron resistance by using evolutionary engineering strategy that was previously applied successfully. Boron-resistant S. cerevisiae mutants were obtained and their phenotypic and physiological characteristics were determined. In order to identify the molecular mechanisms implicated in boron resistance, the whole transcriptomes and genome sequence analysis of wild type and one of the most resistant mutants were compared.
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| Overall design |
BA8 resistant Saccharomyces cerevisiae (BA8) mutant strain and reference strain were grown in yeast minimal medium. When cultures’ Optical Density (OD600) reached to about 1, which correspond to the logarithmic growth phase of the yeast cells, total RNA isolation was carried out by using RNeasy Mini Kit (Qiagen). The experiment was performed triplicates. Whole-genome microarray analysis was carried out by using Agilent DNA Microarray Technology.
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| Contributor(s) |
YILMAZ U, ALKIM C, ARSLAN M, KISAKESEN HI, CAKAR ZP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 07, 2023 |
| Last update date |
Feb 13, 2023 |
| Contact name |
Halil ibrahim KISAKESEN |
| E-mail(s) |
kisakesenhi@gmail.com
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| Phone |
+905307831229
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| Organization name |
Istanbul Technical University
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| Department |
Molecular Biology, Genetics and Biotechnology
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| Lab |
Yeast
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| Street address |
ITU MOBGAM, SARIYER
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| City |
Istanbul |
| State/province |
Istanbul |
| ZIP/Postal code |
34469 |
| Country |
Turkey |
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| Platforms (1) |
| GPL16244 |
Agilent-016322 Yeast (V2) Gene Expression 8x15K Microarray (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA932432 |
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