|
| Status |
Public on May 05, 2023 |
| Title |
The MMP-2 histone H3 N-terminal tail protease is selectively targeted to the transcription start sites of active genes [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Our study examines the role of MMP-2 mediated Histone H3NT proteolysis in U2OS cells. We find binding of MMP-2 at all active protein coding transcription start sites, and examine the role it plays in local chromatin regulation and changes in gene expression.
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| |
|
| Overall design |
native ChIP-seq of 3XHA tagged Pro-MMP2 as well as a negative control (HA ChIP in WT cells not expressing tagged Pro-MMP2) and positive control (H3K4me3)
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| |
|
| Contributor(s) |
Weekley BH, Rice JC |
| Citation(s) |
37161413 |
| |
| Submission date |
Feb 08, 2023 |
| Last update date |
Aug 07, 2023 |
| Contact name |
Benjamin H Weekley |
| E-mail(s) |
benjamin.weekley@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Neuroscience
|
| Lab |
Maze Lab, Hess 9-201
|
| Street address |
1470 Madison Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE224887 |
The MMP-2 histone H3 N-terminal tail protease is selectively targeted to the transcription start sites of active genes |
|
| Relations |
| BioProject |
PRJNA934553 |
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