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Series GSE224891 Query DataSets for GSE224891
Status Public on Feb 09, 2024
Title MSH7 confers quantitative variation in pollen fertility and boosts grain yield in maize
Organism Zea mays
Experiment type Expression profiling by high throughput sequencing
Summary Fertile pollen is critical for the survival, fitness and dispersal of flowering plants, and directly contributes to crop productivity. Extensive mutational screening studies have been carried out to dissect the genetic regulatory network determining pollen fertility, but we still lack fundamental knowledge about whether and how pollen fertility is controlled in natural populations. We used a genome-wide association study (GWAS) to show that ZmGEN1A and ZmMSH7, two DNA repair-related genes, confer natural variation in maize pollen fertility. Mutants defective in both genes exhibited abnormalities in meiotic or post-meiotic DNA repair, leading to reduced pollen fertility. More importantly, ZmMSH7 underwent selection during maize domestication, and its disruption resulted in a substantial increase in grain yield and protein content for both inbred and hybrid. Overall, our study describes the first systematic examination of natural genetic effects on pollen fertility in plants, providing valuable genetic resources for optimizing male fertility. Moreover, ZmMSH7 may be a potential candidate for simultaneous improvement of grain yield and quality.
 
Overall design RNA-seq liabraries were prepared using developing maize seeds (7 DAP and 14 DAP) of zmmsh7-2 and wild-type plants
 
Contributor(s) Jiang L
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Submission date Feb 09, 2023
Last update date Feb 09, 2024
Contact name Luguang Jiang
Organization name China Agricultural University
Street address No.2 Yuanmingyuan West Road, Haidian, Beijing
City Beijing
State/province Beijing
ZIP/Postal code 100193
Country China
 
Platforms (1)
GPL25410 Illumina NovaSeq 6000 (Zea mays)
Samples (12)
GSM7035117 MSH7-WT-AA-7DAP-rep1
GSM7035118 MSH7-WT-AA-7DAP-rep2
GSM7035119 MSH7-WT-AA-7DAP-rep3
Relations
BioProject PRJNA933005

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE224891_MSH7-14DAP-genecounts.txt.gz 422.5 Kb (ftp)(http) TXT
GSE224891_MSH7-7DAP-genecounts.txt.gz 427.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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