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| Status |
Public on Sep 20, 2012 |
| Title |
Minimal peroxide exposure of neuronal cells induces multifaceted adaptive responses. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Oxidative exposure of cells occurs naturally and may be associated with cellular damage and dysfunction. Protracted low level oxidative exposure can induce accumulated cell disruption, affecting multiple cellular functions. Accumulated oxidative exposure has also been proposed as one of the potential hallmarks of the physiological/pathophysiological aging process. We investigated the multifactorial effects of long-term minimal peroxide exposure upon SH-SY5Y neural cells to understand how they respond to the continued presence of oxidative stressors. We show that minimal protracted oxidative stresses induce complex molecular and physiological alterations in cell functionality. Upon chronic exposure to minimal doses of hydrogen peroxide, SH-SY5Y cells displayed a multifactorial response to the stressor. To fully appreciate the peroxide-mediated cellular effects, we assessed these adaptive effects at the genomic, proteomic and cellular signal processing level. Combined analyses of these multiple levels of investigation revealed a complex cellular adaptive response to the protracted peroxide exposure. This adaptive response involved changes in cytoskeletal structure, energy metabolic shifts towards glycolysis and selective alterations in transmembrane receptor activity. Our analyses of the global responses to chronic stressor exposure, at multiple biological levels, revealed a viable neural phenotype in-part reminiscent of aged or damaged neural tissue. Our paradigm indicates how cellular physiology can subtly change in different contexts and potentially aid the appreciation of stress response adaptations.
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| Overall design |
SH-SY5Y cells were maintained at 37oC in a humidified 5% CO2 incubator in AMEM/F12 supplemented with 10% FBS, 100 units/ml ?penicillin and 100 units/ml streptomycin. Cells were treated with either phosphate-buffered saline (PBS) (CTR), or 10nM hydrogen peroxide(CMP) (H2O2: Sigma Aldrich, St Louis MO) for seven days. Cell growth media and applied hydrogen peroxide treatments were changed daily. Both control and hydrogen peroxide treated SH-SY5Y cell were serum-starved overnight prior to ligand stimulations with either, Beta-methylcholine(BMC) (Calbiochem, Gibbstown NJ) or 10ng/ml brain derived neurotrophic factor (BDNF) (Invitrogen, Carlsbad CA) for 2, 4, or 8 hours. Cells were then collected and used to generate total RNA.
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| Contributor(s) |
Chadwick W, Zhou Y, Wang L, Park S, Mitchell N, Stone MD, Becker KG, Martin B, Maudsley S |
| Citation(s) |
21179406 |
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| Submission date |
Jun 23, 2010 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Supriyo De |
| Organization name |
NIA-IRP, NIH
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| Department |
Laboratory of Genetics and Genomics
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| Lab |
Computational Biology & Genomics Core
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| Street address |
251 Bayview Blvd
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| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA128595 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22525_RAW.tar |
6.2 Mb |
(http)(custom) |
TAR |
| GSE22525_non-normalized.txt.gz |
21.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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